FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434567
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23500.5407681669339826No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14970.34448082804262636No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAAAACCCGTG13640.3138756509352988No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13270.30536142873250843No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13030.29983869000637414No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12750.2933954948258842No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12670.2915545819171727No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTTCGGGTCACCATATT12500.28764264198616096No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC12270.2823500173736156No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10990.25289541083423267No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG10050.23126468415687337No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT9350.21515669620564837No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9220.21216521272899228No Hit
GCTCAGAACACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA9030.20779304457080264No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8950.20595213166209123No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATCAGAGGACAAACGGGCAAC8760.20157996350390156No Hit
GAATATCAACATTGAGATTCCCGAGATTGAGATCTTTTGCGACGCGGCGA8720.20065950704954588No Hit
GAATATTAACATTGAGATTCCCGAGATTGAGATCTTTTGCGACGCGGCGA8710.2004293929359569No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8510.19582711066417838No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAACTTCAT8260.19007425782445514No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7900.18179014973525373No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG7800.17948900859936442No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7180.16522193355685083No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCATCAACAACC7000.16107987951225014No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6960.1601594230578944No Hit
ATATTAACGTTGACATAGTTAACTACTAATTCCCTGGATGCTTGGTCCAA6720.15463668433176012No Hit
GTTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAAAGT6650.1530258855366376No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6560.15095485851433726No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6530.15026451617357048No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGGAAAGAAGGAGTTT6500.1495741738328037No Hit
CCCATAGGTATTTTGCGAAAGCCCATGATGATGGGATGGGAATACAGGTG6500.1495741738328037No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6470.1488838314920369No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6460.14865371737844796No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG6440.14819348915127012No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6430.14796337503768117No Hit
GACATAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATGTTGTAC6360.14635257624255868No Hit
GTATTATCCAGAGCATGTAGTTACTCATTACTTCCAGACGCGACATTATT6330.1456622339017919No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6320.14543211978820297No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6240.14359120687949153No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6210.14290086453872475No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6180.14221052219795796No Hit
TACACACTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGT6120.1408298375164244No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6030.13875881049412403No Hit
GCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC6000.13806846815335724No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5990.13783835403976832No Hit
GAGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACA5960.13714801169900154No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5940.1366877834718237No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5930.13645766935823475No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5910.13599744113105688No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5910.13599744113105688No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5900.13576732701746796No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5880.1353070987902901No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG5880.1353070987902901No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTAATCTCTTTTTTGCCTTCTG5720.13162527297286725No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5720.13162527297286725No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5610.12909401772338902No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5510.12679287658749974No Hit
CTGTAAGGGGCCCTACAAATTGCTCACATCTATCAACAATGTCCTCCTGC5470.12587242013314404No Hit
GGCTGAGACAGTGTTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCAC5450.12541219190596617No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA5450.12541219190596617No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5380.12380139311084368No Hit
ATATCAACATTGAGATTCCCGAGATTGAGATCTTTTGCGACGCGGCGATT5370.12357127899725473No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5350.12311105077007689No Hit
GTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA5300.12196048020213224No Hit
GTATCGGGAGGCCTTAGAATCTCCGGAACATTGTTCACCTCACCATACGG5270.12127013786136547No Hit
GAGTATGTAAATAATGTCGCGTCTGGAAGTAATGAGTAACTACATGCTCT5270.12127013786136547No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5220.12011956729342081No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5160.11873888261188723No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATC5160.11873888261188723No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA5120.1178184261575315No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT5110.1175883120439426No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5090.11712808381676473No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT5060.11643774147599795No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA5050.11620762736240901No Hit
GGAAAGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT5030.11574739913523116No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA5010.1152871709080533No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTG4990.11482694268087545No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGGAAAGAAGGAGT4990.11482694268087545No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4980.11459682856728652No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4980.11459682856728652No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4960.11413660034010867No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4940.1136763721129308No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4900.11275591565857508No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4870.11206557331780831No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4820.11091500274986366No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGAAACAGTGAGCCCTGCTCAGA4800.1104547745226858No Hit
CCTGTATTCCCATCCCATCATCATGGGCTTTCGCAAAATACCTATGGGAG4770.109764432181919No Hit
GACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATGTTGTAC4690.10792351927320758No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATACCCCCATCTTTTTGTTT4680.10769340515961866No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4650.10700306281885186No Hit
TCTGTGGAGTTAATCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4620.10631272047808507No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4620.10631272047808507No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4590.1056223781373183No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4520.1040115793421958No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4490.10332123700142902No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGACAAACTGTTCT4490.10332123700142902No Hit
ATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG4480.10309112288784009No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4460.10263089466066222No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4440.10217066643348437No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTTCGGGTCACCATATTCT4420.1017104382063065No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4400.10125020997912865No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG4400.10125020997912865No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4400.10125020997912865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)