FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434567
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21730.5000379688287422No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAAAACCCGTG15610.3592081313123178No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA14910.34310014336109274No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13250.3049012005053306No Hit
GTTAATATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC11880.2733755669436474No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11620.2673925999903352No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11450.2634806600593234No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11380.2618698612642009No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTTCGGGTCACCATATT11320.26048917658266735No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11010.25335563906141056No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAAGCCCCCATG9890.22758285833945052No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT9330.21469646797847053No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8650.19904870825442336No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATCAGAGGACAAACGGGCAAC8510.19582711066417838No Hit
GAATATTAACATTGAGATTCCCGAGATTGAGATCTTTTGCGACGCGGCGA8450.1944464259826448No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8350.1921452848467555No Hit
GAATATCAACATTGAGATTCCCGAGATTGAGATCTTTTGCGACGCGGCGA8350.1921452848467555No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG8210.18892368725651051No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8120.18685266023421016No Hit
GCTCAGAACACTGTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGA7700.17718786746347515No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGGTATTCCCTTAACTTCAT7480.1721253569645187No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG7120.16384124887531726No Hit
ATATTAACGTTGACATAGTTAACTACTAATTCCCTGGATGCTTGGTCCAA7000.16107987951225014No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6900.15877873837636083No Hit
GTTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCACCAGCAGGAAAGT6850.15762816780841618No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA6810.15670771135406047No Hit
GTATTATCCAGAGCATGTAGTTACTCATTACTTCCAGACGCGACATTATT6640.1527957714230487No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6620.15233554319587084No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATCATCAACAACC6350.14612246212896976No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6310.14520200567461403No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6300.1449718915610251No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT6220.14313097865231367No Hit
CCCATAGGTATTTTGCGAAAGCCCATGATGATGGGATGGGAATACAGGTG6190.14244063631154688No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6180.14221052219795796No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6170.14198040808436904No Hit
GTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA6020.1385286963805351No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6010.1382985822669462No Hit
GACATAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATGTTGTAC5950.1369178975854126No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5920.13622755524464583No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5910.13599744113105688No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5900.13576732701746796No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTAATCTCTTTTTTGCCTTCTG5890.13553721290387905No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5870.13507698467670118No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5800.13346618588157866No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5780.13300595765440082No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5740.1320855012000451No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA5730.13185538708645617No Hit
GTATCGGGAGGCCTTAGAATCTCCGGAACATTGTTCACCTCACCATACGG5660.13024458829133367No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5610.12909401772338902No Hit
GAGTATGTAAATAATGTCGCGTCTGGAAGTAATGAGTAACTACATGCTCT5610.12909401772338902No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5590.12863378949621115No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGGAAAGAAGGAGTTT5570.12817356126903331No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5530.1272531048146776No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5530.1272531048146776No Hit
CTGTAAGGGGCCCTACAAATTGCTCACATCTATCAACAATGTCCTCCTGC5400.12426162133802152No Hit
GCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC5400.12426162133802152No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5350.12311105077007689No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5320.12242070842931009No Hit
TCTTATGTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGG5260.12104002374777653No Hit
GGCTGAGACAGTGTTCTGAGCAGGGCTCACTGTTTCTGAACTGGAGCCAC5170.11896899672547616No Hit
TACACACTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGT5150.1185087684982983No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA5140.11827865438470937No Hit
ATATCAACATTGAGATTCCCGAGATTGAGATCTTTTGCGACGCGGCGATT5120.1178184261575315No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTGATTCCAGGATC5120.1178184261575315No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5120.1178184261575315No Hit
GAGGCATAGCAGCAGGATGCAGAGGAAGATGATAAAACGCCGCAGACACA5100.11735819793035367No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5070.11666785558958688No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5030.11574739913523116No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4980.11459682856728652No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGAAACAGTGAGCCCTGCTCAGA4980.11459682856728652No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4950.11390648622651973No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4900.11275591565857508No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4890.11252580154498615No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4870.11206557331780831No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT4860.11183545920421938No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4800.1104547745226858No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4770.109764432181919No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT4720.10861386161397438No Hit
GGAAAGTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGT4720.10861386161397438No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4660.10723317693244079No Hit
CATCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGGAAAGAAGGAGT4640.10677294870526295No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCTGAGACAGTG4620.10631272047808507No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4620.10631272047808507No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGACAAACTGTTCT4610.10608260636449614No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT4580.10539226402372937No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA4510.10378146522860687No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4500.10355135111501793No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG4500.10355135111501793No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT4460.10263089466066222No Hit
ATGTTGTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAG4380.10078998175195078No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)