FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487784

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487784
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395278
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC21810.5517635689312332No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG19580.4953475781601809No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC18980.4801683878181938No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACACACT17400.4401965199176276No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA17190.434883803297932No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16670.42172850500154324No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCCGG12700.32129286223872816No Hit
CCTCCCGGTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11500.2909344815547539No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10520.2661418039961748No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCACTGCTAG9790.24767378908009044No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9720.24590288354019196No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9550.24160211294329562No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT9380.23730134234639924No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT9140.2312296662096044No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8950.22642292260130845No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8800.22262812501581164No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC8380.21200269177642064No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8200.2074489346738245No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7820.1978354474572326No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7710.19505259589453497No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG7590.19201675782613756No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTGCTGG7400.1872100142178416No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7370.18645105470074227No Hit
GTATAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7370.18645105470074227No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCACAG7350.1859450816893427No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG7330.1854391086779431No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7200.1821502841038459No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC7080.17911444603544846No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTG7040.17810250001264932No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG7000.17709055398985019No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6920.17506666194425188No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6920.17506666194425188No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT6820.17253679688725404No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6710.1697539453245564No Hit
GTTTAAATGTATACCCAAAGACAAAAGGAAATTGGTAACAGCGGTAAAAA6660.16848901279605746No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6560.1659591477390596No Hit
CTCCTACCTGGTGTGCTGCTGGCCATTGATCCTTGTTGGGGTTGAAATCC6500.1644412287048609No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCC6430.16267032316496238No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6370.16115240413076365No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6240.15786357955666644No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6150.15558670100536837No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG6130.1550807279939688No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6020.15229787643127116No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6000.1517919034198716No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5980.15128593040847202No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5900.14926203836287374No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGTATAGGCCCCT5810.14698515981157564No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5790.14647918680017608No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5750.14546724077737694No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5650.1429373757203791No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5650.1429373757203791No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5650.1429373757203791No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5640.1426843892146793No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5630.14243140270897953No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGGTTACAGACCACCAAATGCC5620.14217841620327973No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5610.14192542969757993No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5600.14167244319188013No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCCTCAT5590.14141945668618036No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5560.140660497169081No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5520.13964855114628186No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAGATGAATG5520.13964855114628186No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5500.1391425781348823No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5480.13863660512348272No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATCTGAAAG5290.13382986151518678No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5280.13357687500948698No Hit
GGGTAAAGGTTCAGATATTGTTTATACAGAAAGGCCTTGTAAGTTGGCGA5220.13205895597528827No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5220.13205895597528827No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5220.13205895597528827No Hit
GTTCTATCCCAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5200.1315529829638887No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTTTACAATATGTT5170.13079402344678936No Hit
AGGTAGGAGTGGGATCCTTCGGGCCAGGGTTCACTCCACCACACGGCAAT5120.12952909091829043No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5110.12927610441259063No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC5100.12902311790689086No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA5090.12877013140119106No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5080.1285171448954913No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5050.1277581853783919No Hit
GCCTAATCATCTCATGTTCATGTCTCACTGTTCAAGCCTCCAAGCTGTGC5020.12699922586129256No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCT4970.12573429333279362No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGAGGCATGGACATTGA4950.12522832032139405No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4930.12472234730999449No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4910.12421637429859492No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4890.12371040128719535No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAGATGAATGTGAGG4890.12371040128719535No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG4880.12345741478149556No Hit
CTCTGGGATCTAGCAGTGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG4860.12295144177009598No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4800.12143352273589728No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4790.12118053623019748No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4740.11991560370169854No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4700.1189036576788994No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG4700.1189036576788994No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA4680.11839768466749984No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4670.11814469816180005No Hit
GGTATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTGCTG4660.11789171165610027No Hit
ATCCCAGAGTGAGGGGCCTATACCTTCCTGCTGGTGGCTCCAGTTCAGGA4650.11763872515040048No Hit
GGTTTAAATGTATACCCAAAGACAAAAGGAAATTGGTAACAGCGGTAAAA4650.11763872515040048No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4640.1173857386447007No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCCTCATTTTGTGG4590.11612080611620176No Hit
GCCTAGTCATCTCATGTTCATGTCTCACTGTTCAAGCCTCCAAGCTGTGC4570.1156148331048022No Hit
ATCCCAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4570.1156148331048022No Hit
GGCCTATACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGT4550.11510886009340263No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4550.11510886009340263No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4540.11485587358770284No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA4500.11384392756490368No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGT4480.11333795455350411No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4470.11308496804780432No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4460.11283198154210454No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4450.11257899503640476No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4440.11232600853070497No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC4440.11232600853070497No Hit
GCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCTCAGGCCATGC4440.11232600853070497No Hit
CTCCACCACACGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATA4400.11131406250790582No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4380.11080808949650625No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4360.11030211648510668No Hit
GTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCACCTTATGAGTCCAA4350.1100491299794069No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTCCTT4350.1100491299794069No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4330.10954315696800733No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4320.10929017046230755No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4310.10903718395660776No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCAGCACATT4290.10853121094520819No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4290.10853121094520819No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4270.10802523793380862No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4220.10676030540530967No Hit
CCTATAAATAGACCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGGCT4200.1062543323939101No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4170.10549537287681074No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGC4150.10498939986541117No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4140.10473641335971139No Hit
GATTAACTGTGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4120.10423044034831182No Hit
ATATAAGAGAGAAACTACTCGTAGCGCCTCATTTTGTGGGTCACCATATT4090.10347148083121246No Hit
GTGGTAATGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4080.10321849432551268No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4050.10245953480841333No Hit
GTCCAACTGGTGATCGGGAAGGAATCCCAGAGGATTGGGAACAGAAAGAT4030.10195356179701376No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4020.10170057529131397No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)