FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences395278
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG21810.5517635689312332No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC20910.5289947834182525No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACACACT18040.45638765628241384No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC17630.4460152095487227No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16560.41894565343884554No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT15650.3959238814201651No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTGTGTTCCGG14290.36151771664499416No Hit
CCTCCCGGTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11490.29068149504905405No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT10020.2534924787111855No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9640.2438789914945937No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9310.2355304368065007No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCACTGCTAG9140.2312296662096044No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT8990.22743486862410758No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8890.22490500356710974No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8670.21933930044171443No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8510.21529151635051785No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8370.21174970527072087No Hit
GTATAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC8320.2104847727422219No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7610.19252273083753713No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7490.1894868927691397No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC7380.18670404120644205No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACATTACCACAG7300.18468014916084377No Hit
GTTTAAATGTATACCCAAAGACAAAAGGAAATTGGTAACAGCGGTAAAAA7180.18164431109244633No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6990.17683756748415042No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTTTTTCCTTCTATTCG6960.17607860796705102No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG6940.17557263495565145No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6870.17380172941575295No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6670.16874199930175723No Hit
CTCCTACCTGGTGTGCTGCTGGCCATTGATCCTTGTTGGGGTTGAAATCC6580.16646512075045916No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6550.16570616123335982No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6540.16545317472766002No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTG6540.16545317472766002No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTGCTGG6460.16342928268206175No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6230.15761059305096667No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6170.15609267401676796No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6020.15229787643127116No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5980.15128593040847202No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5950.15052697089137265No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5910.14951502486857352No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5890.14900905185717395No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCC5810.14698515981157564No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5740.14521425427167714No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG5740.14521425427167714No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAGGTATAGGCCCCT5740.14521425427167714No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAGATGAATG5740.14521425427167714No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5700.144202308248878No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5690.14394932174317823No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5650.1429373757203791No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT5650.1429373757203791No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5640.1426843892146793No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG5620.14217841620327973No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGGTTACAGACCACCAAATGCC5570.1409134836747808No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5540.14015452415768143No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG5490.1388895916291825No Hit
GTTCTATCCCAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5400.13661271307788442No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5360.13560076705508528No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5320.13458882103228612No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5270.1333238885037872No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5260.1330709019980874No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGAGGCATGGACATTGA5220.13205895597528827No Hit
GGGTAAAGGTTCAGATATTGTTTATACAGAAAGGCCTTGTAAGTTGGCGA5170.13079402344678936No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5120.12952909091829043No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5070.1282641583897915No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCTTTA5030.12725221236699233No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5000.126493252849893No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4970.12573429333279362No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4940.12497533381569427No Hit
ATCCCAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4910.12421637429859492No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4910.12421637429859492No Hit
ATCCCAGAGTGAGGGGCCTATACCTTCCTGCTGGTGGCTCCAGTTCAGGA4910.12421637429859492No Hit
GCCTAATCATCTCATGTTCATGTCTCACTGTTCAAGCCTCCAAGCTGTGC4890.12371040128719535No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTTTACAATATGTT4880.12345741478149556No Hit
GCCTACTCCCATCTCTCCACCTCTAAGAGACAGTCATCCTCAGGCCATGC4860.12295144177009598No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCT4850.1226984552643962No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4850.1226984552643962No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCCTCAT4820.12193949574729684No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4820.12193949574729684No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4730.11966261719599876No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4730.11966261719599876No Hit
GGCCTATACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGT4730.11966261719599876No Hit
GCCTAGTCATCTCATGTTCATGTCTCACTGTTCAAGCCTCCAAGCTGTGC4720.11940963069029897No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC4710.11915664418459919No Hit
CTCTGGGATCTAGCAGTGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG4700.1189036576788994No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC4660.11789171165610027No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAGATGAATGTGAGG4620.11687976563330112No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4590.11612080611620176No Hit
GCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCCTCATTTTGTGG4580.11586781961050198No Hit
GGTATAGGCCCCTCACTCTGGGATCTAGCAGTGCTTGGTGGAATGTGCTG4580.11586781961050198No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4560.11536184659910241No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4540.11485587358770284No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4530.11460288708200306No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4490.11359094105920389No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4490.11359094105920389No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4470.11308496804780432No Hit
CTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACA4460.11283198154210454No Hit
GGTTTAAATGTATACCCAAAGACAAAAGGAAATTGGTAACAGCGGTAAAA4430.11207302202500519No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4420.1118200355193054No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA4410.11156704901360562No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT4400.11131406250790582No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATCTGAAAG4360.11030211648510668No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4310.10903718395660776No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4290.10853121094520819No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4290.10853121094520819No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4280.10827822443950841No Hit
AGGTAGGAGTGGGATCCTTCGGGCCAGGGTTCACTCCACCACACGGCAAT4280.10827822443950841No Hit
CTCCACCACACGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATA4260.10777225142810884No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC4220.10676030540530967No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT4210.10650731889960989No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4190.10600134588821032No Hit
GATTAACTGTGTGTTCCGGATAATAAGGTTTAATGCCTTTATCTAAGGGC4180.10574835938251054No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG4170.10549537287681074No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGCGC4150.10498939986541117No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4120.10423044034831182No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4120.10423044034831182No Hit
ATATAAGAGAGAAACTACTCGTAGCGCCTCATTTTGTGGGTCACCATATT4080.10321849432551268No Hit
ACCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA4080.10321849432551268No Hit
GTCCAACTGGTGATCGGGAAGGAATCCCAGAGGATTGGGAACAGAAAGAT4070.1029655078198129No Hit
CCTATAAATAGACCTATTGATTGGAAAGTGTGTCAACGAATTGTGGGGCT4070.1029655078198129No Hit
GTGGTAATGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4060.10271252131411311No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTCCTT4050.10245953480841333No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA4030.10195356179701376No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGT4020.10170057529131397No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT4010.10144758878561419No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG3980.10068862926851482No Hit
CTCCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAATATG3960.10018265625711524No Hit
CCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAACTCCAGCACATT3960.10018265625711524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)