FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487788

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487788
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477191
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA26250.55009419708251No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT20500.4295973729596744No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT18000.3772074494280068No Hit
TCTACGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG15970.33466683152029275No Hit
TCTTCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG13940.2921262136125786No Hit
CATTTAAACCCTACCAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT13700.2870967809535385No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG13090.27431363961181165No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAATACATAAATC12990.2722180426705449No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12610.26425477429373145No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC11960.25063339417549785No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11070.2319825813982242No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT10770.2256957905744241No Hit
CCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACCTTACCACAG9420.1974052318673236No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC8930.18713680685511672No Hit
GTACCAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT8810.18462209052559667No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT8770.18378385174908998No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGTAAAAA8710.18252649358432996No Hit
ATGTCAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC8590.18001177725480993No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG8440.17686838184290987No Hit
CCCATCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCC8370.17540146398402318No Hit
GCACGGGACCATGCAAGAACTGCACGACTCCTGCTCAAGGAACCTCTATG8250.17288674765450313No Hit
GTTAACATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTG8220.1722580685721231No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG7870.16492347927768966No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7820.1638756808070563No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7810.16366612111292964No Hit
GTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC7780.16303744203054962No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7530.15779844967738285No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7330.15360725579484943No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7320.15339769610072276No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG7010.146901345582796No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6940.1454344277239093No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6910.14480574864152929No Hit
CTACTATTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG6820.14291971139438925No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6770.1418719129237559No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6760.14166235322962922No Hit
AATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT6720.14082411445312254No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6690.14019543537074253No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6610.13851895781772916No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6520.13663292057058915No Hit
GTCCGGCAGATGTGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6510.13642336087646248No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG6510.13642336087646248No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATGACATATCCC6490.13600424148820914No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTTCTTTTCCTCAC6480.13579468179408244No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6390.1339086445469424No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT6360.13327996546456242No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTTTGCCAAGTGTTTGCTG6350.13307040577043575No Hit
TCATTAATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAG6330.1326512863821824No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATGACATATCCCATGAA6290.13181304760567572No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG6250.13097480882916904No Hit
GTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAACAAG6220.13034612974678902No Hit
TGCAAGAACTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6170.12929833127615567No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6140.12866965219377566No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC6090.1276218537231423No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6060.1269931746407623No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6050.12678361494663562No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT6030.12636449555838228No Hit
GGTTTAAGTCCCAATCTGGATTGTTTGAATTGGCTCCGAATGCAGGGTCC5930.12426889861711558No Hit
CCTTTTTTCGTTAACTGTAAGAGGGCCTACATATTGTTGACATCTATTGA5920.12405933892298891No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT5880.12322110014648222No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG5860.12280198075822889No Hit
GAGCTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAGAGCAGAG5840.12238286136997555No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5820.1219637419817222No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTGGTAGGGTTTAAATGTATA5810.12175418228759552No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGATGGGTCTTCC5800.12154462259346888No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5740.12028726442870884No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGTAAAA5730.12007770473458218No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA5720.11986814504045551No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5680.11902990626394881No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGTTCTTGCATGGTCCCGTG5660.11861078687569547No Hit
GTTCATCTCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAG5630.11798210779331547No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5530.11588651085204875No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG5530.11588651085204875No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5510.11546739146379544No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5490.11504827207554208No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5450.11421003329903538No Hit
CTACTAATTCCCTGGATGATGGGTCTTCCAAATTACTTCCCACCCAGGTG5410.11337179452252871No Hit
CCTACCGCTTACCGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5340.11190487666364202No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTC5340.11190487666364202No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTTTGCCAAGTG5330.11169531696951535No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5320.11148575727538867No Hit
GACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTAGTTTTGGTAG5190.10876148125174197No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCACATCTGCCGGACCGTGTG5150.1079232424752353No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5140.10771368278110861No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5120.10729456339285527No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5100.10687544400460193No Hit
GTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGC5100.10687544400460193No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5050.10582764553396858No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5030.10540852614571523No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG5030.10540852614571523No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC4990.10457028736920856No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4960.10394160828682854No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4920.10310336951032187No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4910.1028938098161952No Hit
CCACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAG4900.10268425012206853No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4870.1020555710396885No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4840.1014268919573085No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4830.10121733226318183No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4790.10037909348667516No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)