FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487789

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487789
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477191
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA21380.4480386260428214No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19900.4170237913120742No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17470.36610078563929327No Hit
TCTACGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG14760.30931010853096563No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG13690.28688722125941185No Hit
TCTTCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG13610.2852107437063985No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAATACATAAATC13420.28122910951799174No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12710.2663503712349981No Hit
CATTTAAACCCTACCAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT12660.2653025727643648No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11970.2508429538696245No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT9700.20327290330287034No Hit
CCCTTAACTTCATGGGATATGTCATTGGAAGTTGGGGTACCTTACCACAG9520.19950082880859027No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC9070.1900706425728901No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT8650.18126913541956993No Hit
GTACCAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT8600.1802213369489366No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACGAGAAAAACCCC8600.1802213369489366No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8220.1722580685721231No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT8070.16911467316022305No Hit
GCACGGGACCATGCAAGAACTGCACGACTCCTGCTCAAGGAACCTCTATG8040.16848599407784304No Hit
ATGTCAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC7910.16576171805419632No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGTAAAAA7870.16492347927768966No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7780.16303744203054962No Hit
CCCATCATCCAGGGAATTAGTAGTAAGCTATGTCAATGTTAATATGGGCC7670.16073228539515624No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7420.15549329304198947No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAG7380.15465505426548282No Hit
GTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC7310.15318813640659612No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7200.1508829797712027No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6970.1460631068062893No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT6970.1460631068062893No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6870.1439675098650226No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATGACATATCCCATGAA6770.1418719129237559No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG6720.14082411445312254No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGAGCATGGACATTGA6620.13872851751185583No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG6570.13768071904122248No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTTTGCCAAGTGTTTGCTG6560.1374711593470958No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6540.13705203995884246No Hit
GTTAACATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCCTG6450.13516600271170245No Hit
CTACTATTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG6380.13369908485281573No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6380.13369908485281573No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC6310.13223216699392903No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6290.13181304760567572No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6260.1311843685232957No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT6240.13076524913504237No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCTTCTTTTCCTCAC6210.13013657005266235No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6170.12929833127615567No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATGACATATCCC6140.12866965219377566No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6040.12657405525250895No Hit
GTTCATCTCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAG6010.12594537617012896No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6000.12573581647600227No Hit
GTCCGGCAGATGTGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5970.12510713739362225No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCACATCTGCCGGACCGTGTG5920.12405933892298891No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5900.12364021953473556No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5890.12343065984060889No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTC5780.12112550320521552No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5770.12091594351108885No Hit
TGCAAGAACTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5760.12070638381696218No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5750.12049682412283551No Hit
CCTTTTTTCGTTAACTGTAAGAGGGCCTACATATTGTTGACATCTATTGA5750.12049682412283551No Hit
GGTTTAAGTCCCAATCTGGATTGTTTGAATTGGCTCCGAATGCAGGGTCC5730.12007770473458218No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5680.11902990626394881No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG5660.11861078687569547No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTCACAGCGGTAAAA5620.1177725480991888No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGATGGGTCTTCC5600.11735342871093545No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5530.11588651085204875No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5510.11546739146379544No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5500.11525783176966875No Hit
AATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTT5460.11441959299316207No Hit
TCATTAATCTATTCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAG5450.11421003329903538No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5420.11358135421665538No Hit
GAGCTGAACAATGTTCCGGAGACTCTAAGGCCTCCCGATACAGAGCAGAG5390.11295267513427537No Hit
CTTTAATCCTGACTGGCAAACTCCTTCTTTTCCTCACATTCATTTGAAAG5340.11190487666364202No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5330.11169531696951535No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTGGTAGGGTTTAAATGTATA5330.11169531696951535No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCAGGTCTTTGCCAAGTG5320.11148575727538867No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5300.11106663788713533No Hit
GTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAACCAACAAG5250.11001883941650198No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5160.10813280216936194No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG5130.10750412308698194No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5060.10603720522809526No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5030.10540852614571523No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5000.10477984706333524No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4950.10373204859270187No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4920.10310336951032187No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4920.10310336951032187No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA4880.10226513073381517No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGTTCTTGCATGGTCCCGTG4860.10184601134556184No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4820.10100777256905516No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA4810.10079821287492849No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)