FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487791

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487791
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244045
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG19980.8187014689913745No Hit
CCTCCCGCATACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17720.7260955971234814No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10570.43311684320514654No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT9550.39132127271609746No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG9400.3851748652912373No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8510.3487061812370669No Hit
GTATTCATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC7440.30486180827306436No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7420.304042287283083No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7240.2966665983732508No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTATCTCCTCC7080.29011043045339996No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCAGG6950.2847835440185212No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6690.2741297711487635No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACAT6230.2552807883791924No Hit
AATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC6180.25323198590423895No Hit
GTATACATGCATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAA6060.24831485996435085No Hit
ATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5940.24339773402446271No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5920.24257821303448135No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT5900.24175869204449998No Hit
GTATACATTTGAATCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC5860.24011965006453728No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTTCG5820.23848060808457455No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT5680.23274396115470505No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5670.23233420065971438No Hit
GGGTTCACCCCTCCACACGGAGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5640.23110491917474238No Hit
CATACAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5590.22905611669978898No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5550.22741707471982628No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5380.22045114630498472No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT5340.21881210432502202No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC5340.21881210432502202No Hit
GTCAACATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTGTGG5090.20856809195025508No Hit
GTATAATATGATCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4840.19832407957548812No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA4810.19709479809051608No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4780.19586551660554408No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4740.19422647462558135No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC4700.19258743264561864No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4620.1893093486856932No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT4600.18848982769571185No Hit
CATATAAAGGCATCAGAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4560.18685078571574915No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4560.18685078571574915No Hit
GTACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4440.181933659775861No Hit
GGATCATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAA4410.18070437829088898No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4390.1798848573009076No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4370.17906533631092625No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4260.1745579708660288No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4130.16923108443114998No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAGATTAATTATGCCTGCTAGG4090.16759204245118728No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCA4030.1651334794812432No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4000.16390419799627118No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTACTGGTGGTTGATGTTCCTGG3960.16226515601630848No Hit
CCTAGTACAAAGACCTTTAGCCTAATCTCCTCCCCCAACTCCTCCCAGTC3930.16103587453133644No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3930.16103587453133644No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT3900.15980659304636438No Hit
GAGGTGTATTTGCGAGAAAGGACAACAGAGTTGTCAGTCCCGATAAGTTT3890.1593968325513737No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3760.1540699461164949No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA3750.15366018562150424No Hit
GTAGAGCCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3630.1487430596816161No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA3500.14341617324673728No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG3490.1430064127517466No Hit
ATATTATACAAAAACTCAAGCAATGTTTTCGAAAACTGCCTGTAAATAGA3450.14136737077178388No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC3440.1409576102767932No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3410.13972832879182118No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3340.13686000532688644No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG3340.13686000532688644No Hit
AATGAATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAAGGGAC3300.1352209633469237No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC3220.1319428793869983No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3210.13153311889200764No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3200.13112335839701694No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA3190.13071359790202627No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3100.12702575344711017No Hit
GTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCA3080.1262062324571288No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3080.1262062324571288No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3060.12538671146714744No Hit
GAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCC3050.12497695097215676No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA3030.12415742998217542No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3020.12374766948718476No Hit
GTCTATTTACAGGCAGTTTTCGAAAACATTGCTTGAGTTTTTGTATAATA3000.12292814849720339No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2990.12251838800221271No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT2960.12128910651724067No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC2960.12128910651724067No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2960.12128910651724067No Hit
CTTCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT2950.12087934602224999No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG2920.11965006453727796No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG2870.11760126206232456No Hit
CATACTGCGGAACTCCTGGCAGCTTGTTTTGCTCGCAGCCGGTCTGGAGC2860.11719150156733389No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT2860.11719150156733389No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2840.11637198057735253No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2820.11555245958737119No Hit
GCATATTGACAACAGTGCCAGCTGCGCCTCCTCCTGCCTCCACCAATCGG2690.11022557315249236No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2670.10940605216251101No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCCATTCGAGATCTCCTCGACACC2670.10940605216251101No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCTCCGTG2620.10735724968755761No Hit
ATTGACAACAGTGCCAGCTGCGCCTCCTCCTGCCTCCACCAATCGGCAGT2610.10694748919256696No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCATACAGACCACCAAATGCC2610.10694748919256696No Hit
GCCTACGGCCAAGCCCCATCCAGTGGGGGTTGCGTCAGCAAACACTTGGC2610.10694748919256696No Hit
GGATAGAACCTAGCAGGCATAATTAATCTTAATCTCCTTTTTTCATTAAC2590.1061279682025856No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC2570.10530844721260424No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG2570.10530844721260424No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG2570.10530844721260424No Hit
GCACACCCGAGCAGCCATGGGAAGGAGGTGTATTTGCGAGAAAGGACAAC2560.10489868671761356No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2560.10489868671761356No Hit
ACTGCATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTA2550.10448892622262287No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC2550.10448892622262287No Hit
CTTTTACACAATGTGGCTATCCTGCTCTGATGCCTTTATATGCATGTATA2540.1040791657276322No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC2520.10325964473765084No Hit
ACTTATCGGGACTGACAACTCTGTTGTCCTTTCTCGCAAATACACCTCCT2520.10325964473765084No Hit
GTATGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA2470.10121084226269744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)