FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487792

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487792
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367934
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18230.4954692961237613No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14080.3826773279990433No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATTTGGCCAC10180.2766800567493083No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG9990.2715160871243212No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTACGCCTCA9410.25575239037436065No Hit
ATTGACAACAGTGCCAACAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9050.24596802687438507No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8800.23917332999940205No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7960.21634314849945913No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGC7930.21552778487446117No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGT7910.21498420912446253No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7630.20737414862448156No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7500.2038409062494904No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT7350.19976408812450058No Hit
GCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTT7220.1962308457495094No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7100.19296939124951756No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA7000.19025151249952438No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATTTGGCCAC6910.18780542162453048No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6890.18726184587453185No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6870.1867182701245332No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6860.1864464822495339No Hit
TAGCCAACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCAC6650.18073893687454817No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6570.1785646338745536No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA6550.17802105812455496No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6490.17639033087455902No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6480.1761185429995597No Hit
CTTCAGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATT6470.17584675512456038No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC6470.17584675512456038No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6390.17367245212456583No Hit
GCCTTTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTT6280.1706827854995733No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA6190.1682366946245794No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6140.16687775524958282No Hit
GCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCC5920.16089842199959775No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAGTCTTTA5830.15845233112460388No Hit
CCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTACGCCTCAA5740.15600624024961No Hit
AGTTAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGT5740.15600624024961No Hit
AATAAAGGCCCCTCACTCTGGGATCTAGCAGAGTTTGGTGAAATGTGGTG5740.15600624024961No Hit
GGTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCCC5690.1546473008746134No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5660.1538319372496154No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5610.15247299787461882No Hit
CCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAAC5520.15002690699962493No Hit
AATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGAAATGTGGTG5470.14866796762462833No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5350.14540651312463648No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5320.14459114949963853No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTTAGA5110.13888360412465278No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5000.13589393749966025No Hit
GCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAG4970.13507857387466232No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4900.13317605874966706No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4860.1320889072496698No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4860.1320889072496698No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4840.13154533149967113No Hit
GGATAAGGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4740.12882745274967794No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4730.12855566487467862No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4670.12692493762468268No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4620.1255659982496861No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4610.12529421037468677No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4500.12230454374969424No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4460.12121739224969696No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4410.11985845287470036No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4360.11849951349970375No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4340.11795593774970511No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4330.11768414987470578No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4330.11768414987470578No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4320.11741236199970648No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4310.11714057412470714No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4230.1149662711247126No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4220.11469448324971326No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4180.11360733174971599No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4170.11333554387471666No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4090.11116124087472211No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC4050.1100740893747248No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4010.10898693787472753No Hit
GGAACATATTGTACTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4000.1087151499997282No Hit
CTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATT3960.10762799849973093No Hit
GTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGCGTCCAAGGAAT3920.10654084699973365No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC3890.10572548337473568No Hit
GCTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT3870.10518190762473704No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCT3840.10436654399973908No Hit
AATATAGGCCCCTCACTCTGGGATCTAGCAGAGTTTGGTGAAATGTGGTG3770.10246402887474383No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG3760.10219224099974453No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3750.1019204531247452No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3720.10110508949974722No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC3720.10110508949974722No Hit
TGGAAACCACCGTGAACGCCCATCAGGTCTTGCCCAAGGTCTTACATAAG3680.10001793799974995No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)