FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487793

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487793
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367934
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18240.4957410839987606No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12660.3440834497491398No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10090.2742339658743144No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATTTGGCCAC9390.25520881462436196No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA8270.22476857262443808No Hit
ATTGACAACAGTGCCAACAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8160.22177890599944555No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTACGCCTCA8090.21987639087445032No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7850.21335348187446662No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7500.2038409062494904No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT7070.1921540276245196No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7050.19161045187452097No Hit
GCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTT6780.18427217924953931No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6770.18400039137454No Hit
GCCTTTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTT6680.1815543004995461No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6620.1799235732495502No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA6610.17965178537455087No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6540.1777492702495556No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACCGAGT6440.17503139149956243No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6440.17503139149956243No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTAATGAATTTGGCCAC6390.17367245212456583No Hit
TAGCCAACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCAC6330.1720417248745699No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACATAAATC6260.17013920974957464No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6220.16905205824957736No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA6200.16850848249957873No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6120.16633417949958415No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT6080.16524702799958688No Hit
CTTCAGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATT5890.1600830583745998No Hit
GCCTTAGTGCTGTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCC5840.15872411899960318No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGCCCGGC5750.1562780281246093No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5730.15573445237461067No Hit
GGTCCTAGGAATCCTGATGTTGTGTTCTCCATGTTCGGTGCAGGGTCCCC5620.15274478574961814No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5520.15002690699962493No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5520.15002690699962493No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5520.15002690699962493No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5350.14540651312463648No Hit
AATAAAGGCCCCTCACTCTGGGATCTAGCAGAGTTTGGTGAAATGTGGTG5220.1418732707496453No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5200.14132969499964668No Hit
AGTTAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATTGT4970.13507857387466232No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTTAGA4860.1320889072496698No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGGCCGTGTG4780.12991460424967521No Hit
AATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGAAATGTGGTG4760.12937102849967658No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4750.12909924062467726No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA4720.1282838769996793No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4700.12774030124968064No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4690.12746851337468135No Hit
GGATAAGGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4640.12610957399968473No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4540.12339169524969151No Hit
CCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTACGCCTCAA4510.12257633162469357No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4500.12230454374969424No Hit
GCCTTTATCCAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAG4490.1220327558746949No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4420.12013024074969966No Hit
CCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAGCCAGGAGAAAC4400.11958666499970103No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4370.11877130137470308No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC4360.11849951349970375No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4300.11686878624970784No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT4290.11659699837470851No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4180.11360733174971599No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4170.11333554387471666No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4160.11306375599971735No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4140.11252018024971869No Hit
CTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATT4140.11252018024971869No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4110.11170481662472075No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGTCCGACCT4100.11143302874972141No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4100.11143302874972141No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG4080.11088945299972278No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4050.1100740893747248No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4040.1098023014997255No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4020.10925872574972686No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCGACTCCTCCCAGTCTTTA3990.1084433621247289No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3880.10545369549973638No Hit
TGGAAACCACCGTGAACGCCCATCAGGTCTTGCCCAAGGTCTTACATAAG3850.10463833187473841No Hit
GTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA3840.10436654399973908No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3840.10436654399973908No Hit
GTGTTGGGGTGAGTTAATGAATTTGGCCACCTGGGTGGGAAGTAATTTGG3710.10083330162474792No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3710.10083330162474792No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC3700.10056151374974859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)