FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487794

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487794
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421726
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16960.40215685065658746No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA15770.37393947729094246No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14260.3381342388185694No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG13850.3284122866505741No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12900.3058858121149751No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12440.29497825602405353No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT12200.2892873571940075No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10970.2601215006900215No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10430.24731697832241786No Hit
ATTTACATACTCTGTGGCAGGCGGGCATTTTATATAAGAGAGAAACTACA10120.23996623400027506No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9800.23237836889354702No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9510.22550186614057466No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9300.2205223296642844No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8730.20700644494292503No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8730.20700644494292503No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG8700.2062950825891693No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8580.20344963317414627No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8410.19941857983619699No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA8390.19894433826702645No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8270.19609888885200344No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8200.19443904335990667No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7720.18305724569981457No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7560.17926331314645053No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7110.16859287784011417No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG7090.1681186362709437No Hit
TTTACATACTCTGTGGCAGGCGGGCATTTTATATAAGAGAGAAACTACAC7090.1681186362709437No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6850.16242773744089764No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6840.1621906166563124No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6670.15815956331836312No Hit
GTGTGTAGTTTCTCTCTTATATAAAATGCCCGCCTGCCACAGAGTATGTA6550.15531411390334007No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6450.15294290605748756No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6270.148674731934953No Hit
GTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG6180.14654064487368576No Hit
GCACGGGACCATGCAAGACTTGCACGACTCCTGCTCAAGGAACCTCTATG6140.14559216173534476No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6140.14559216173534476No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAAGTCTTGCATGGTCCCGTG5880.1394270213361282No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5800.13753005505944618No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGAGGTG5780.13705581349027568No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5770.13681869270569044No Hit
AGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA5740.13610733035193467No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5730.1358702095673494No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5660.13421036407525264No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5590.1325505185831559No Hit
TTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA5500.13041643152188861No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5370.12733386132228033No Hit
AAACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAA5350.12685961975310983No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5310.12591113661476883No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5300.12567401583018356No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5290.12543689504559832No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT5230.12401417033808683No Hit
CTCTGTGGCAGGCGGGCATTTTATATAAGAGAGAAACTACACACAGCGCT5220.12377704955350156No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCCGCCTGCCACAGAGTATGTAAA5210.12353992876891631No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5180.12282856641516056No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5140.12188008327681954No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5120.12140584170764904No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5070.12022023778472278No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5060.11998311700013753No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5040.11950887543096703No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT4990.11832327150804077No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4960.11761190915428502No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT4950.11737478836969976No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4950.11737478836969976No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4930.11690054680052926No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTATAGACCACCAAATGCC4920.116663426015944No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4920.116663426015944No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4920.116663426015944No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4910.11642630523135875No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4890.11595206366218824No Hit
CTTATATAAAATGCCCGCCTGCCACAGAGTATGTAAATAATGCCTAGTTT4890.11595206366218824No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4810.11405509738550623No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT4800.11381797660092098No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4780.11334373503175048No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4780.11334373503175048No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4770.11310661424716521No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA4760.11286949346257996No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4740.11239525189340946No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4730.11215813110882422No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4710.1116838895396537No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4650.1102611648321422No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4620.10954980247838644No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4600.10907556090921594No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4570.10836419855546019No Hit
GCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4560.10812707777087492No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4550.10788995698628968No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4530.10741571541711917No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4520.10717859463253393No Hit
GTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATAC4510.10694147384794866No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4500.10670435306336341No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4480.10623011149419291No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4480.10623011149419291No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4470.10599299070960767No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4430.10504450757126665No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4430.10504450757126665No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4340.10291042050999939No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC4300.10196193737165837No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4240.10053921266414687No Hit
GTCTATAAGCGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAC4220.10006497109497636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)