FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487795

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487795
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences421726
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16970.40239397144117267No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12710.3013805172078553No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12300.29165856503986004No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12270.29094720268610424No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11650.2762457140418186No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10980.26035862147460676No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9670.22929579869393873No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9490.22502762457140418No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9210.2183882426030171No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8700.2062950825891693No Hit
CCTCCCGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8570.203212512389561No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8320.19728449277492968No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT8130.1927791978678099No Hit
ATTTACATACTCTGTGGCAGGCGGGCATTTTATATAAGAGAGAAACTACA8080.19159359394488365No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7970.18898526531444587No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7790.18471709119191132No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT7770.18424284962274082No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7590.1799746755002063No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7530.17855195079269479No Hit
CCTACTCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7460.176892105300598No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7130.1690671194092847No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA6980.16551030764050592No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6920.1640875829329944No Hit
GTGTGTAGTTTCTCTCTTATATAAAATGCCCGCCTGCCACAGAGTATGTA6880.16313909979465338No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6780.16076789194880087No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT6760.1602936503796304No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6570.15578835547251058No Hit
GCACGGGACCATGCAAGACTTGCACGACTCCTGCTCAAGGAACCTCTATG6550.15531411390334007No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6440.15270578527290232No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6320.14986033585787928No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6020.14274671232032174No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6020.14274671232032174No Hit
GTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG5980.1417982291819807No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5740.13610733035193467No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5730.1358702095673494No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC5700.13515884721359367No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGAGGTG5640.13373612250608216No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5570.1320762770139854No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5440.1289937068143771No Hit
GGGTAAAGGTGCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5370.12733386132228033No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5360.1270967405376951No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5340.1266224989685246No Hit
TTTACATACTCTGTGGCAGGCGGGCATTTTATATAAGAGAGAAACTACAC5330.12638537818393933No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5320.1261482573993541No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG5290.12543689504559832No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5280.12519977426101306No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5220.12377704955350156No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5170.1225914456305753No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAAGTCTTGCATGGTCCCGTG5050.11974599621555226No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5000.11856039229262601No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4980.11808615072345552No Hit
CATTTGAACCCTAATAAAACCAAACGATGGGGCTACTCCCTTAACTTCAT4980.11808615072345552No Hit
GTGTAGTTTCTCTCTTATATAAAATGCCCGCCTGCCACAGAGTATGTAAA4950.11737478836969976No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4890.11595206366218824No Hit
AGTTAAGGGAGTAGCCCCATCGTTTGGTTTTATTAGGGTTCAAATGTATA4890.11595206366218824No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG4870.11547782209301774No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTATAGACCACCAAATGCC4830.11452933895467673No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA4790.11358085581633572No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4760.11286949346257996No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4760.11286949346257996No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4710.1116838895396537No Hit
CTTATATAAAATGCCCGCCTGCCACAGAGTATGTAAATAATGCCTAGTTT4690.1112096479704832No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4630.1097869232629717No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4610.10931268169380118No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4580.10860131934004544No Hit
GTCCAACTGGTGATCGGGAAAGAATCCCAGAGGATTGGGAACAGCAAGAT4530.10741571541711917No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT4530.10741571541711917No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA4510.10694147384794866No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4510.10694147384794866No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4470.10599299070960767No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4440.10528162835585189No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4440.10528162835585189No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4430.10504450757126665No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGCCG4410.10457026600209614No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4400.1043331452175109No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4390.10409602443292565No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4390.10409602443292565No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4370.10362178286375515No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4350.10314754129458464No Hit
CTCTGTGGCAGGCGGGCATTTTATATAAGAGAGAAACTACACACAGCGCT4340.10291042050999939No Hit
TTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA4340.10291042050999939No Hit
CTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACT4320.10243617894082889No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4320.10243617894082889No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4320.10243617894082889No Hit
GCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4310.10219905815624362No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4270.10125057501790262No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGTGTGTAGTTTCTCTCTTATA4260.10101345423331735No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA4250.10077633344873212No Hit
GGGTTCACCCCACCTCACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4250.10077633344873212No Hit
AAACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAA4240.10053921266414687No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4230.1003020918795616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)