FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences700042
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN30880.44111639015944754No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT24550.35069324411963854No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGCATACATTTAA22980.32826601832461483No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18300.2614128866553721No Hit
TTCAAGAACAGTGTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA18270.2609843409395436No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT17880.2554132466337734No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA17810.25441330663017364No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA17270.246699483745261No Hit
AGTCTAGACTCTGTGGTATTGTTAGGATTTTTGTCAACGAGAAAAACCCC16950.24212832944309057No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTTCCAAAAGTAAGA16370.23384311227040663No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16320.2331288694106925No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT15430.22041534650778094No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14810.21155873504732572No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGAGACACCCGTG12990.18556029495373133No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT12900.1842746578062459No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT12830.18327471780264612No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC12590.1798463520760183No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTTGATCGTC12560.17941780636018984No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC12400.1771322292091046No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG12360.1765608349213333No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTACGGGTCACCATATT12330.1761322892055048No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12210.1744181063421909No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG12200.17427525777024808No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG11980.1711325891875059No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC11730.16756137488893522No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT11470.16384731201842176No Hit
ATATGTTCCTGTGGTAATGTGCCCCAACTCCCAATTATATATCCCATGAA10950.1564191862773948No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10770.15384791198242392No Hit
CAATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG10710.15299082055076693No Hit
GTACAACATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTTTGTC9910.14156293479534085No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA9900.14142008622339802No Hit
ATATAATTGGGAGTTGGGGCACATTACCACAGGAACATATTGTACAAAAA9590.13699178049317043No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGG9500.13570614334568498No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATACGGGAGTAGGCTGCCT9230.13184923190322867No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT9040.12913510903631498No Hit
GTTGTGAGTAGGCCCTGAGACTGAGGGCTCCACCCCAACAGTCCCCCATG9010.1287065633204865No Hit
GAAATGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATG8960.12799232046077236No Hit
GTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTTTTGCCTTCTG8930.12756377474494388No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT8930.12756377474494388No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC8810.12584959188162997No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATATCACCATACGG8770.12527819759385866No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC8730.12470680330608735No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATATCACCATACGGCACTCAGGCA8590.12270692329888779No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG8450.12070704329168822No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8440.12056419471974539No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT8350.11927855757225994No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG8350.11927855757225994No Hit
CTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTT8210.11727867756506037No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC8200.11713582899311756No Hit
GTGGTAATGTGCCCCAACTCCCAATTATATATCCCATGAAGTTAAGGGAA8040.11485025184203235No Hit
ATTATATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTGTG7960.11370746326648973No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAGAT7920.11313606897871843No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7870.11242182611900428No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7850.11213612897511864No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGATAAAGGGATCAAAC7850.11213612897511864No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7810.11156473468734733No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7770.11099334039957602No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGG7750.11070764325569038No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGCGGAGTTACTCTCTTTT7520.10742212610100536No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG7490.1069935803851769No Hit
CCTCTATGTTTCCCTCATGTTGTTGTACAAAACCTACGGACGGAAACTGC7490.1069935803851769No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7400.10570794323769145No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC7390.10556509466574863No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAGGAGACTA7360.10513654894992014No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA7290.10413660894632037No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATTCCTATGGGAG7290.10413660894632037No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA7230.10327951751466341No Hit
CTCTAAGGCCTCCCGATACAGAGCAGCGGCGGTGTCGAGGAGATCTCGAA7220.10313666894272058No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7220.10313666894272058No Hit
GTTCAGAGCCAACTCAGAAAATCCAGATTGGGACCTCAACCCGCACAAGG7180.10256527465494927No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGACACTGTTCT7180.10256527465494927No Hit
CCTCTAAGGGACACTCATCCTCAGGCCGTGCAGTGGAACTCCACCACTTT7170.10242242608300646No Hit
GAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGGACGATCAAGC7150.10213672893912079No Hit
GTTTAAATGTATGCCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAAA7150.10213672893912079No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG7120.10170818322329232No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAGTCTCCTGTTTTCATTTAC7100.10142248607940667No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA7080.10113678893552101No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)