FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259477
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA9380.36149639467081857No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCGACACTTCCGGA8720.3360606142355585No Hit
TTCACAAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA7910.3048439746104664No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7770.29944850603329004No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCGCCTGCCTCCACCAATCGGCAGT7100.2736273349853744No Hit
GGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTT6830.2632217884436771No Hit
ATTCTGTGCTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAA6570.2532016325146352No Hit
GTTCTTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC6420.24742077332480333No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT6310.24318147658559333No Hit
ATATCTGCCCTTAGATAAAGGAATCAAACCGTATTATCCAGAGTATGTAG6270.2416399141349715No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6020.23200514881858508No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTCACTTCAT5640.2173603055376777No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG5610.21620413369971134No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5560.21427718063643406No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5410.2084963214466022No Hit
GTTTGTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC5400.20811093083394674No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5360.2065693683833249No Hit
ATATTAACGTTGACATAGCTGACTACTGCTTCCCTGGATGTTGGATCGTC5270.2031008528694258No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5220.20117389980614853No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5150.19847616551756034No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5040.1942368687783503No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4960.19115374387710665No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4720.18190436917337566No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4550.1753527287582329No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4540.17496733814557744No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4450.17149882263167834No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC4380.16880108834309013No Hit
TTCAAGAACAGTTTGTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA4220.16263483854060282No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4140.1595517136393592No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4110.15839554180139281No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4100.15801015118873735No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4100.15801015118873735No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT3920.15107312016093913No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC3870.14914616709766185No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3770.14529226097110726No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3700.14259452668251907No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3620.1395114017812754No Hit
TCCTCAGGCCATGCAGTGGAACGCCACCACTTTCCACCAAACTCTTCAAG3560.13719905810534266No Hit
GGCAGATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTT3480.134115933204099No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3440.13257437075347717No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACATCACCATACGG3390.1306474176901999No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCCGTG3380.13026202707754445No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG3370.12987663646488898No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC3370.12987663646488898No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3370.12987663646488898No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3370.12987663646488898No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTCAGTAAACAGTATC3360.12949124585223354No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACGCCACCACTTT3360.12949124585223354No Hit
ATATTTGCCCTTAGATAAAGGAATCAAACCGTATTATCCAGAGTATGTAG3340.12872046462692263No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3340.12872046462692263No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3330.12833507401426716No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT3320.1279496834016117No Hit
GAATTCGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTT3300.1271789021763008No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3240.12486655850036804No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3230.12448116788771259No Hit
ATCCAACATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGC3190.12293960543709077No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA3190.12293960543709077No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3170.12216882421177985No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA3140.12101265237381348No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA3120.12024187114850257No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT3120.12024187114850257No Hit
CCCGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAA3120.12024187114850257No Hit
CCTGCATATAGACCACCAAATGCCCCTATCTTATCGACACTTCCGGAAAC3070.11831491808522528No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA3050.11754413685991436No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC3030.11677335563460346No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGCAAACTGC3030.11677335563460346No Hit
CCCAATTACATATCCCATGAAGTGAAGGGAATATCCCCATCGTTTTGTTT3020.11638796502194798No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA2990.11523179318398162No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT2940.11330484012070435No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT2930.11291944950804889No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT2930.11291944950804889No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA2890.11137788705742706No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA2840.10945093399414978No Hit
CCCCTATCTTATCGACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGC2820.10868015276883886No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2820.10868015276883886No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT2810.1082947621561834No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC2810.1082947621561834No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT2790.10752398093087248No Hit
CTATTCTGTGCTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGT2780.10713859031821703No Hit
GGGTAAAGGTTCAGATACTGTTTACTGAGAAAGGCCTTGTAAGTTGGCGA2690.10367007480431792No Hit
ACACACAATAGCTTGCCTGAGCGCCGTATGGTGATGTGAACAATGTTCCG2690.10367007480431792No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC2660.10251390296635153No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT2640.10174312174104064No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA2610.10058694990307426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)