FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences259477
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCGACACTTCCGGA9450.3641941289594068No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8230.31717647421544104No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7250.2794081941752063No Hit
GTTCTTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC6940.26746108518288714No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6880.26514874150695433No Hit
GGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTT6790.26168022599305524No Hit
TTCACAAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA6770.26090944476774436No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCGCCTGCCTCCACCAATCGGCAGT6590.2539724137399461No Hit
ATATCTGCCCTTAGATAAAGGAATCAAACCGTATTATCCAGAGTATGTAG6460.24896233577542518No Hit
ATTCTGTGCTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAA5900.22738046146671959No Hit
GTTTGTGAATATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCTGC5630.21697491492502227No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5620.2165895243123668No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5550.2138917900237786No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5480.2111940557351904No Hit
ATATTAACGTTGACATAGCTGACTACTGCTTCCCTGGATGTTGGATCGTC5330.20541319654535853No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5280.20348624348208127No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT5080.19577843122897212No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTCACTTCAT4890.18845600958851846No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC4860.18729983775055206No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT4770.18383132223665297No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4760.1834459316239975No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4530.174581947532922No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4410.1699572601810565No Hit
GCACGGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG4400.16957186956840106No Hit
CTTTCACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4390.1691864789557456No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4240.16340561976591372No Hit
TTCAAGAACAGTTTGTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA4120.15878093241404825No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4040.1556978075128046No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4000.15415624506218278No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3920.15107312016093913No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC3700.14259452668251907No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACATCACCATACGG3690.1422091360698636No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3660.14105296423189723No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT3600.1387406205559645No Hit
GTCCCGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3550.1368136674926872No Hit
TCCTCAGGCCATGCAGTGGAACGCCACCACTTTCCACCAAACTCTTCAAG3550.1368136674926872No Hit
ATCCAACATCCAGGGAAGCAGTAGTCAGCTATGTCAACGTTAATATGGGC3490.13450132381675448No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC3440.13257437075347717No Hit
GGCAGATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAATTAATTT3370.12987663646488898No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3330.12833507401426716No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA3320.1279496834016117No Hit
ATATTTGCCCTTAGATAAAGGAATCAAACCGTATTATCCAGAGTATGTAG3320.1279496834016117No Hit
CTTTTACGCAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA3300.1271789021763008No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT3280.12640812095098988No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT3260.12563733972567895No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG3210.12371038666240167No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3200.12332499604974623No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT3200.12332499604974623No Hit
ATGTATCTGCGGCGTTTTATCATCTTCCTCTGCATCCTGCTGCTATGCCT3180.1225542148244353No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3180.1225542148244353No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACGCCACCACTTT3180.1225542148244353No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC3140.12101265237381348No Hit
GAATTCGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTT3090.1190856993105362No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3070.11831491808522528No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGCAAACTGC3020.11638796502194798No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3010.11600257440929254No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3000.11561718379663709No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3000.11561718379663709No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG2990.11523179318398162No Hit
TTCAAGAACAGTTTCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA2990.11523179318398162No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT2980.11484640257132617No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT2950.11369023073335979No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG2920.11253405889539343No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2900.11176327767008251No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT2890.11137788705742706No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC2830.10906554338149432No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2810.1082947621561834No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTCAGTAAACAGTATC2780.10713859031821703No Hit
CCCAATTACATATCCCATGAAGTGAAGGGAATATCCCCATCGTTTTGTTT2780.10713859031821703No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC2730.10521163725493975No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCCGTG2720.1048262466422843No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGTAATTTGG2720.1048262466422843No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA2720.1048262466422843No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC2710.10444085602962883No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA2680.10328468419166245No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA2670.10289929357900701No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA2620.10097234051572972No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2610.10058694990307426No Hit
CTATTCTGTGCTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAGGT2610.10058694990307426No Hit
CCCCAGCACAGAATAGCTTGCCTGAGTGCCGTATGGTGATGTGAACAATG2610.10058694990307426No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)