FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487805

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487805
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences335306
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCCGG15880.47359725146582526No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC15400.45928196930564913No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8920.2660256601432721No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT8640.25767507888316943No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8530.2543944933881291No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8320.24813155744305201No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8300.24753508735304466No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8230.24544744203801902No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8200.24455273690300797No Hit
GTACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7740.23083392483283927No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC7710.22993921969782827No Hit
GTGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAGC7480.2230798136627439No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6990.20846629645756412No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAT6530.1947474843873954No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT6510.19415101429738807No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6480.19325630916237707No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6420.19146689889235505No Hit
GGATATTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6380.19027395871234037No Hit
GTCCCGTACTGGTGGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5790.17267809105712392No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5540.16522221493203224No Hit
CCTATCGCTTACAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA5510.16432750979702124No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGACGATGGGATGGGAATACAAG5500.16402927475201756No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5500.16402927475201756No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA5440.16223986448199554No Hit
GGATACTAACATTGGGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5390.1607486892569772No Hit
GTGCTGGTGGACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5380.16045045421197354No Hit
GGAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTAGCTTGTATA5130.15299457808688183No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG4930.14702987718680846No Hit
GTCTTACATAAGCGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4920.1467316421418048No Hit
GTTTTGAAGTAATGATTAACTACATGTTCCGGATAATAAGGTTTAATGCC4920.1467316421418048No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAA4920.1467316421418048No Hit
GGAACATATTGTACAAAAACTCAAGCAATGTTTTCGCAAACTTCCTGTAA4910.14643340709680114No Hit
ATGTAAGACCTTGGGCAAGGCTTGGTGGGCGTTCACGGTGGTCTCCATGC4800.14315282160176077No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4700.1401704711517241No Hit
CAATATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4700.1401704711517241No Hit
CTTTTACACAGTGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA4550.13569694547666905No Hit
CAATTTGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4540.1353987104316654No Hit
AGCGATAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGGA4490.13390753520664708No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACACTGTGTAAAA4470.13331106511663973No Hit
AGGCCTTCCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAA4460.13301283007163606No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT4440.13241635998162873No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA4400.13122341980161403No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTATTGATAATGTCCTCCTGC4370.13032871466660303No Hit
CTCTAACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4360.13003047962159936No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACA4300.12824106935157736No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4190.12496048385653702No Hit
TTCTTCTACTGTACCTGTTTTTAATCCTGAGTGGCAAACTCCCTCCTTTC4160.12406577872152601No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA4130.12317107358651501No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTACATGTTCC4100.12227636845150398No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4100.12227636845150398No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4030.12018872313647833No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG3920.11690813764143797No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGTCCGTGTG3760.11213637692137927No Hit
ACCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT3730.11124167178636828No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC3720.1109434367413646No Hit
ATTTACAGGAAGTTTGCGAAAACATTGCTTGAGTTTTTGTACAATATGTT3700.11034696665135728No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAAGTTAAGGGAG3640.10855755638133527No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3580.10676814611131324No Hit
GATGCAGAGGAATATGATAAAACGCCGCAGACACATCCAACGATAGCCAG3550.10587344097630225No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3470.1034875606162729No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3470.1034875606162729No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3470.1034875606162729No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3460.10318932557126922No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3450.10289109052626558No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3450.10289109052626558No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACCACC3400.10139991530124723No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG3390.10110168025624355No Hit
GGTCTATTTACAGGAAGTTTGCGAAAACATTGCTTGAGTTTTTGTACAAT3390.10110168025624355No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT3370.10050521016623622No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)