FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477358
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC19890.41666841238651076No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16940.3548699299058568No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14780.30962087154714074No Hit
ATCCTAACTGTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAC14310.29977501162649417No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC12440.2606010583251983No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT11950.250336225641971No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT11460.24007139295874375No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG11310.23692909723938846No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10590.2218460777864831No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9560.2002689805135768No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAACATAAGA9440.1977551439380926No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT9210.1929369571684145No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT8850.18539544744196182No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC8590.17994880152841264No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG8440.17680650580905735No Hit
ATCTTAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG8430.176597019427767No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8390.1757590739026056No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8230.17240729180195993No Hit
CCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG8230.17240729180195993No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8160.1709408871329275No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG8110.16989345522647573No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA8090.16947448246389502No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA7950.1665416731258301No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC7450.15606735406131247No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA7380.15460094939228No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7180.15041122176647298No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7160.14999224900389224No Hit
TATTAACATTGACATAGCCGACTACTAATTCTCTGGATGCTGGATCTTCC6840.14328868480260099No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGT6790.14224125289614922No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG6740.14119382098969746No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT6700.14035587546453607No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6690.1401463890832457No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6660.13951792993937465No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT6630.1388894707955036No Hit
GTCCAAGAGTCCGCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC6520.13658512060130973No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6450.13511871593227726No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6370.13344282488195444No Hit
CTCCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6360.13323333850066407No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6360.13323333850066407No Hit
ATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAACCTG6290.1317669338316316No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6290.1317669338316316No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6180.12946258363743773No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6040.12652977429937282No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6000.1256918287742114No Hit
CTCAAGGACAGTTTCTCTTCCAAACATAAGACAAGAAATGTGAAACCACA6000.1256918287742114No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5840.12234004667356574No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA5840.12234004667356574No Hit
CTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG5810.12171158752969469No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTTAT5800.12150210114840435No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA5750.12045466924195257No Hit
GGGTAAAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5730.12003569647937187No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA5690.11919775095421045No Hit
GTTCTATCCTAACTGTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC5660.1185692918103394No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGTAAGACCTTGGG5600.11731237352259728No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5590.11710288714130695No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA5580.11689340076001659No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5570.11668391437872623No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5560.11647442799743589No Hit
GTCCAGAAGAACCCACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5440.11396059142195167No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5390.11291315951549989No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5340.11186572760904813No Hit
TACCTTGATAGTCCAGAAGAACCCACAAGAAGATGAGGCATAGCAGCAGG5260.11018983655872532No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG5250.10998035017743496No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCT5240.10977086379614462No Hit
GGATTAAAGATAGGTATGGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5220.10935189103356391No Hit
GTGTTGGGGTGAGTTGATGAACCTGGCCACCTGGGTGGGAAGTAATTTGG5210.10914240465227357No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5200.10893291827098321No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCGCG5190.10872343188969284No Hit
GTGGTAAAGTACCCCAACTCCCAATCACATATCCCATAAAGTTAAGGGAG5170.10830445912711215No Hit
GATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGCAGCCGGTCTG5150.10788548636453145No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCGCGCGGCCG5150.10788548636453145No Hit
ATCCCAGAGTGAGGGGCCTGTATTTTCCTGCTGGTGGCTCCAGTTCCGGA5130.10746651360195074No Hit
CCTCTGCACGTCGCATGGCGACCACCGTGAACGCCCACCAGGTCTTGCCC5120.1072570272206604No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG5090.10662856807678933No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATG5080.10641908169549898No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA5070.10620959531420862No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACCA5060.10600010893291828No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5040.10558113617033756No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT5010.1049526770264665No Hit
CTTTTGGAGTGTGGATTCGCACACCTCCCGCTTACAGACCACCAAATGCC4920.10306729959485333No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTAGGGCATGGACATTGACCCGTAT4910.10285781321356299No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4870.10201986768840157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)