FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477358
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15690.3286841322445628No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13530.28343507388584666No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG13440.2815496964542335No Hit
ATCCTAACTGTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAC12380.2593441400374562No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG10280.2153519999664822No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8880.18602390658583287No Hit
CTCCAAAAGACACCAAATACTCAAGGACAGTTTCTCTTCCAAACATAAGA8510.17827291047808982No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT8500.17806342409679946No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA8340.17471164199615383No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8200.17177883265808888No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA7920.16591321398195905No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT7760.1625614318813134No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7710.16151399997486163No Hit
ATCTTAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG7650.16025708168711952No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC7570.15858119063679668No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA7370.15439146301098966No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7090.1485258443348598No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7070.1481068715722791No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG6890.14433611670905275No Hit
CTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6820.14286971204002027No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCGCTTATGT6680.13993690270195533No Hit
CCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6530.13679460698260004No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG6480.1357471750761483No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA6150.12883412449356668No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6100.12778669258711492No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG6070.12715823344324387No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA5990.12548234239292103No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCGCGCGGCCG5950.12464439686775963No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5900.12359696496130786No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5800.12150210114840435No Hit
CTCCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5660.1185692918103394No Hit
GTTCTATCCTAACTGTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC5650.11835980542904906No Hit
CTCTTATATAGAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5620.117731346285178No Hit
TATTAACATTGACATAGCCGACTACTAATTCTCTGGATGCTGGATCTTCC5620.117731346285178No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC5490.11500802332840342No Hit
CTGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAG5430.1137511050406613No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5390.11291315951549989No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5270.11039932294001568No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG5190.10872343188969284No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5180.10851394550840252No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5100.10683805445807967No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5010.1049526770264665No Hit
CTTTTGGAGTGTGGATTCGCACACCTCCCGCTTACAGACCACCAAATGCC4970.10411473150130511No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT4960.10390524512001476No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4950.10369575873872439No Hit
GGGTAAAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4890.10243884045098228No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCT4840.10139140854453052No Hit
CCCTTAACTTTATGGGATATGTGATTGGGAGTTGGGGTACTTTACCACAG4820.10097243578194982No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4810.10076294940065947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)