FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487808

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487808
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498537
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTTTATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT21070.4226366347934055No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA16670.33437839117257095No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16250.32595374064512767No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12740.25554773266578007No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11880.23829725777625332No Hit
GTCTCTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA11270.22606145581972856No Hit
CATCTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT10320.2070056986743211No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10190.20439806874916006No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10140.20339513416255964No Hit
TTTGCTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACAC9380.18815052844623367No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT8820.17691766107630927No Hit
ATCCAGCATCCAGGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGC8680.17410944423382818No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT8540.17130122739134707No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8510.17069946663938684No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8020.160870707690703No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT8000.16046953385606286No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG7980.16006836002142266No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7940.15926601235214236No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7830.1570595562616215No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG7760.15565544784038096No Hit
CTCCAAAAGACACCAAATATTCAAGAACTGTTTCTCTCCCAAAAGTAAGA7530.15104194874201915No Hit
GTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATT7430.14903607956881837No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC7330.1470302103956176No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7320.1468296234782975No Hit
GCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA7310.14662903656097742No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7270.1458266888916971No Hit
ATTCATGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCG7270.1458266888916971No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7180.1440214066358164No Hit
GTACCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7160.14362023280117625No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCGGACTTCTTTCCTTCTATTCG6800.13639910377765344No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6680.1339920607698125No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6600.13238736543125185No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6420.12877680091949043No Hit
GGGTAAAGGTTGAGATACTGGTTACGTAAAAAGGCCTTGTAAGTTGGCGA6400.12837562708485029No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6310.12657034482896956No Hit
GCCTAGCAGCCATGGAAATGATGTGTACTTGCGGGAGAGCACGACAGAAT6250.1253668233250491No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGTATTGTGGGTCT6160.12356154106916838No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6000.12035215039204712No Hit
GTTGATAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAAACAG5930.11894804197080658No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC5910.11854686813616643No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG5870.11774452046688612No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5870.11774452046688612No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5780.11593923821100541No Hit
GTACCGAACATGGAGAACATCGCATCAGGACTCCTAGGACCCCTGCTCGT5750.11533747745904517No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT5710.11453512978976485No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5700.11433454287244477No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5560.11152632602996367No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5530.11092456527800344No Hit
TCCATGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5500.1103228045260432No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5460.1095204568567629No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTT5440.10911928302212272No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5390.10811634843552234No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5350.10731400076624202No Hit
CACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTTGC5330.10691282693160187No Hit
CTCTTTGGAAGGCGGGGATATTATATAAAAGAGAGTCCACACGTAGCGCC5280.10590989234500146No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5270.1057093054276814No Hit
TAGATAAAGGGATCAAACCGTATTATCCAGAGCATGTAGTTAATCATTAC5240.10510754467572116No Hit
ATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA5210.10450578392376092No Hit
CTCTTTTATATAATATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5200.10430519700644085No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5140.10310167550252036No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5040.1010958063293196No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTGAGATACTGGTTACGT5040.1010958063293196No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5000.10029345866003929No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)