FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487810

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487810
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474233
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACGGCTTGTTCAGG33400.7042951460568961No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT26380.5562666452988299No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG16910.35657577604257823No Hit
ATCCTAACATTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTAT16210.34181509932881093No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15920.33569996183310735No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15910.3354890950229107No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT14950.31524588124402986No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13890.2928939993631822No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12660.26695738170899114No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCACTTAACTTCAT11890.25072063732384714No Hit
GTTCAGGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTA11870.2502989037034538No Hit
CTCCAAAAGATACCAAATACTCAAGAACAGTTTCTCTCCCAAAAGTAAGA11580.24418376620775017No Hit
CTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA11010.2321643580265397No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10700.22562748691044276No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10590.22330795199827932No Hit
CTGTAAGAGGGCCCACATATTGTTCACATCTATTAATAATGTCCTCCTGT10430.2199340830351325No Hit
ATCCTGAACAAGCCGTTAATCATTACTTCAAAACTAGGCATTATTTACAT10350.2182471485535591No Hit
GTTCTATCCTAACATTACTAAATATTTGCCCTTAGACAAAGGCATTAAAC10200.21508414640060897No Hit
TGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9940.2096016093354954No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9670.20390820546018518No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACGGCT9650.20348647183979185No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACGGCTTGTTCA9190.19378659857074476No Hit
TCCTCAGGCCGTGCAGTGGAACTCCACAACATTCCACCAAGCTCTACTAG9150.19294313132995805No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATAGGAGTAGGCTGTCT9120.19231053089936803No Hit
CCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACAAGCCGTTAATCA9070.19125619684838466No Hit
GGATAATACGGTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAATGTT8870.18703886064445116No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCACCGTG8850.1866171270240578No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGAACAACAGAGT8660.18261065763032097No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8400.17712812056520738No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8380.17670638694481405No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8240.1737542516020606No Hit
TAATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA8140.17164558350009385No Hit
GTCTAAGGGCAAATATTTAGTAATGTTAGGATAGAACCTAGCAGGCATAA7900.1665847800553736No Hit
ACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTATTATCCTGAACA7700.1623674438514401No Hit
GTATTATCCTGAACAAGCCGTTAATCATTACTTCAAAACTAGGCATTATT7660.16152397661065343No Hit
GAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATT7660.16152397661065343No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA7590.16004790893927667No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATATT7400.15604143954553984No Hit
CCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC7360.15519797230475316No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC7280.15351103782317974No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACTAATTTTCTTTTGTC7090.1495045684294429No Hit
ATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA7040.14845023437845953No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6920.14591983265609942No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCGGGCTTACAGACCACCAAATGCC6870.14486549860511605No Hit
GTTTAATGCCTTTGTCTAAGGGCAAATATTTAGTAATGTTAGGATAGAAC6840.14423289817452603No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6750.14233509688275595No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6750.14233509688275595No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC6710.14149162964196924No Hit
GATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTA6580.13875036110941244No Hit
ATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6460.13621995938705236No Hit
GCCTTAGAGTCTCCGGAACATTGTTCAGCTCACCATACAGCACTCAGGCA6410.13516562533606896No Hit
CATTTACAGGAGGACATTATTAATAGATGTGAACAATATGTGGGCCCTCT6340.13368955766469226No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCATCTGGCG6260.13200262318311887No Hit
TTCTTCTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTC6250.13179175637292218No Hit
GTCTATTTACAGGCAATTTTCTAAAACATTGTTTGAGTTTTAGTACAATA6140.12947222146075874No Hit
GATATAGGCCCCTCACTCTGGGATCTAGTAGAGCTTGGTGGAATGTTGTG6040.127363553358792No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6020.12694181973839863No Hit
GTTTTGAAGTAATGATTAACGGCTTGTTCAGGATAATACGGTTTAATGCC5970.12588748568741526No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5960.1256766188772186No Hit
CTACTGTACCTGTCTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCAC5950.1254657520670219No Hit
GTACAATATGTTCCTGCGGTAAAGTACCCCAACTTCCAATTACATATCCC5880.12398968439564517No Hit
CTTTAATCCCGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTACAGG5850.12335708396505515No Hit
GTCTTGGCCCCCAATACCAGATCATCCATATAACTGAAAGCCAAACAGTG5830.1229353503446618No Hit
GGTCTATTTACAGGCAATTTTCTAAAACATTGTTTGAGTTTTAGTACAAT5760.12145928267328508No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5760.12145928267328508No Hit
ATTAATAGATGTGAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG5660.11935061457131832No Hit
CACTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG5660.11935061457131832No Hit
GGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACTAATT5610.11829628052033495No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5520.11639847922856486No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT5510.11618761241836818No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5470.1153441451775815No Hit
ATCTGGTATTGGGGGCCAAGACTGTACAACATCTTGAGTCCCTTTTTACC5400.11386807750620476No Hit
GGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA5350.11281374345522138No Hit
CTGTAAGCCCGAGGAGTGCGAATCCACACTCCAAAAGATACCAAATACTC5290.11154854259404132No Hit
TTACTAAATATTTGCCCTTAGACAAAGGCATTAAACCGTATTATCCTGAA5270.11112680897364796No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5230.11028334173286128No Hit
CTCTAAGGCCTCCCGATACAAAGCAGAGGCGGTGTCGAGGAGATCTCGAA5220.1100724749226646No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5180.10922900768187789No Hit
ACATTGACCCGTATAAAGAATTTGGAGCATCTGGCGAGTTACTCTCTTTT5170.10901814087168121No Hit
GTATAAAGAATTTGGAGCATCTGGCGAGTTACTCTCTTTTTTGCCTTCTG5160.10880727406148455No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5160.10880727406148455No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5160.10880727406148455No Hit
CCCACATATTGTTCACATCTATTAATAATGTCCTCCTGTAAATGAATGTG5150.10859640725128787No Hit
GCTAGGTTCTATCCTAACATTACTAAATATTTGCCCTTAGACAAAGGCAT5120.10796380682069784No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACGG5050.10648773914932112No Hit
GCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA5020.10585513871873109No Hit
GTGCTGGTGGCTGAGTGTGAGGACGGCTTTCTCATGTAGGCTGGGGGCAC4980.10501167147794438No Hit
ATACCAGATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4980.10501167147794438No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4950.10437907104735436No Hit
GAATATGGTGACCCACAAAATGAGGCACTGCGTGTAGTTTCTCTCTTATA4940.10416820423715768No Hit
TATTAACATTGACATAGCCGACTACTAGTTCCCTGGATGCTGGATCTTCC4880.10290300337597762No Hit
CCTCGGGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4850.10227040294538761No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4810.1014269357046009No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG4780.10079433527401087No Hit
GGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGAGGAGGTGC4750.10016173484342086No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)