FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375618
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN31310.8335596270679254No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT15670.4171791554185369No Hit
CAGTATCTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACAATATTTGT11270.30003886927676526No Hit
CTACTACTTCCCTGGATGCTGGATCGTCCAAATTACTTCCCACCCAAGTG10400.27687703997146035No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA10250.2728836211257182No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9840.2619682762806894No Hit
GACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAAAGTTACTAAATAT9440.2513191593587102No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACTTGGGTGGGAAGT9170.24413100543637417No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACTTGGGTGGGAAGTAA8990.2393389028214835No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT8830.23507925605269184No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8720.23215074889914752No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8530.22709241836120742No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAATTCAGACA8210.21857312482362398No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7660.20393058905590253No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG7540.20073585397930876No Hit
ATGCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTTAGGAAAAG7270.19354770005697278No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6900.18369726690414198No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAAAGTAAGA6680.17784025259705338No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT6670.1775740246740039No Hit
CCCTTACAGTGAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG6280.16719113567507415No Hit
CATGTATACAAGCCAAACAGGCTTTTACTTTCTCGCCAACTTACAAAGCC6270.16692490775202465No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACTTT5980.15920429798358973No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC5740.15281482783040215No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5710.15201614406125372No Hit
ACTACTTCCCTGGATGCTGGATCGTCCAAATTACTTCCCACCCAAGTGGC5680.15121746029210528No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT5550.147756497292462No Hit
GATACTGTTGACGTAGAAAGGCTTTGTAAGTTGGCGAGAAAGTAAAAGCC5520.14695781352331358No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAACATGGGCCTAAAAATCAGACA5510.1466915856002641No Hit
TCTCCTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5430.14456176221586825No Hit
CAGTAAGCCCTGCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCG5390.1434968505236703No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTATCTCTCCCAAAAGTAAGA5360.14269816675452188No Hit
CAATCCGTTGACACACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTC5310.1413670271392745No Hit
GGATAATACGGTTTGATCCCTTTATCTAGGGGCAAATATTTAGTAACTTT5260.14003588752402707No Hit
GCACCGGACCATGCAAAACCTGCACAGCTCCTGCTCAAGGAACCTCTATG5040.13417887321693847No Hit
CAGTAAATGAAACCAGGAGACTGAAATTAATTATGCCTGCTAGGTTTTAT5030.13391264529388902No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5020.13364641737083952No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAAATGGCAAACTCCTT5010.13338018944779007No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4900.13045168229424575No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4890.13018545437119627No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4890.13018545437119627No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4800.12778940306375094No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA4720.1256595796793551No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG4690.12486089591020665No Hit
ATACAGCACTCAGGCAAGCTATTGTGTGTTGGGGTGAGTTAATGAATCTA4640.12352975629495923No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4590.12219861667971184No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT4530.12060124914141494No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG4340.1155429186034748No Hit
AATCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT4300.11447800691127688No Hit
GGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCT4300.11447800691127688No Hit
GGCCAAATCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATT4270.11367932314212843No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC4210.11208195560383155No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4210.11208195560383155No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4180.1112832718346831No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC4170.11101704391163364No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAGGGGCAAATA4130.10995213221943571No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4090.10888722052723777No Hit
CAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT4070.1083547646811388No Hit
CCCCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGC4010.10675739714284194No Hit
ATCTAGGGGCAAATATTTAGTAACTTTGGGATAAAACCTAGCAGGCATAA3910.10409511791234713No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT3860.10276397829709971No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA3820.1016990666049018No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT3780.10063415491270386No Hit
CTGTAAGGGGCCCCACAAAATGCTTACATCTATCAACAATGTCCTCCTGC3770.10036792698965438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)