FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487814

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487814
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559736
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18740.33480069175468435No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT17230.3078236883102034No Hit
ATATAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC16980.30335729701144826No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT16060.2869209770320294No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA15130.2703060014006603No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15050.2688767561850587No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA13660.24404362056398016No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA13080.23368159275086825No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC12930.2310017579716152No Hit
CTCCAAAAGACACCAAATATTCAAGAACAACTTCTCTTCCAAAAGTAAGA12260.21903182929095144No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT12140.21688796146754902No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC12070.21563737190389756No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGTCTGGA11640.20795517887003873No Hit
GTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCTGG11610.2074192119141881No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC11360.202952820615433No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA11000.1965212171452256No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT10940.19544928323352437No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA10800.1929481041062215No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG10430.18633784498406392No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATAGCCCATGAA10340.18472994411651206No Hit
CTCACAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTAAC10320.18437263281261165No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA10020.1790129632541055No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCTTAACAGTAAATGAAAACAG9840.17579716151900182No Hit
GTATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG9680.17293867108779853No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT9230.16489916675003932No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA9100.16257664327468663No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA8660.1547157945888776No Hit
GGTGTAAGCAATTTGTGGGACCCTTAACAGTAAATGAAAACAGGAGATTA8640.1543584832849772No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA8600.1536438606771764No Hit
GTCTTATATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8420.15042805894207267No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA8390.14989209198622208No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8240.147212257206969No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8240.147212257206969No Hit
GCTTTATTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT8010.14310317721211427No Hit
ACCCTTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTA8000.14292452156016408No Hit
TTTGCGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACC8000.14292452156016408No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC7850.140244686780911No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7740.13827947460945875No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7670.1370288850458073No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7560.13506367287435506No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTGCTGATTTCTTTCCTTCCGCTCG7550.13488501722240487No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT7520.13434905026655425No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7520.13434905026655425No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAAC7480.1336344276587534No Hit
GACCTCAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTT7450.13309846070290282No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT7430.1327411493990024No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC7410.13238383809510199No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGAGAGCACGACAGAAT7380.13184787113925137No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7350.13131190418340075No Hit
CCCTTATCTCCACCTCTAAGGGACACGCATCCTCAGGCCATACAGTGGAA7280.13006131461974932No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7270.1298826589677991No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG7050.12595223462489458No Hit
ACTACATGCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTA7010.12523761201709377No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6980.12470164506124316No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG6960.12434433375734275No Hit
GCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTA6920.12362971114954194No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6880.1229150885417411No Hit
GTTAAGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGCAA6880.1229150885417411No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG6810.12166449897808967No Hit
CCCAATTACATAGCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT6710.11987794245858763No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC6710.11987794245858763No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6640.11862735289493619No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC6610.11809138593908555No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6480.11576886246373291No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA6470.1155902068117827No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6390.11416096159618107No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6380.11398230594423087No Hit
CTCCTAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT6380.11398230594423087No Hit
CTCTTATATAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTC6360.11362499464033045No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC6320.11291037203252961No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATTTACAT6320.11291037203252961No Hit
CTGTTAAGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC6300.1125530607286292No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6290.11237440507667902No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT6290.11237440507667902No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTAAC6250.11165978246887819No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6220.11112381551302757No Hit
CCTTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA6150.10987322594937614No Hit
CCTATGTCAATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTT6120.10933725899352552No Hit
GTATGGATGGGCAGCGGCGACACAAAGGTTCCGCGCATGCGCTGATGGCC6110.10915860334157532No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG6100.1089799476896251No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA6100.1089799476896251No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6060.10826532508182429No Hit
TCCTCAGGCCATACAGTGGAATTCCACCACTTTCCATCAAACTCTTCAAG6030.10772935812597367No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6000.10719339117012305No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAGTTGTTCT5950.10630011291037204No Hit
CCTCTAAGGGACACGCATCCTCAGGCCATACAGTGGAATTCCACCACTTT5920.10576414595452141No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG5870.10487086769477039No Hit
CAATTACATAGCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG5820.10397758943501936No Hit
GAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCT5690.1016550659596667No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGT5680.1014764103077165No Hit
CCAAAAGACACCAAATATTCAAGAACAACTTCTCTTCCAAAAGTAAGACA5680.1014764103077165No Hit
GTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCTG5640.10076178769991569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)