FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences559736
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT19130.34176826218074235No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN18670.3335501021910329No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGT17300.30907427787385483No Hit
GTATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG15500.2769162605228179No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14820.26476767619020397No Hit
GTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA14190.253512370117341No Hit
GTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCTGG14130.2524404362056398No Hit
ATATGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC13870.24779538925493444No Hit
ATATAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC13810.24672345534323326No Hit
TCCAAAGAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACA13460.24047050752497603No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12830.22921520145211313No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA12530.223855531893607No Hit
TTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTC12430.22206897537410492No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATT12410.2217116640702045No Hit
CTCCAAAAGACACCAAATATTCAAGAACAACTTCTCTTCCAAAAGTAAGA12260.21903182929095144No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTTGTCTGGA12180.2176025840753498No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG11800.210813669301242No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC10950.19562793888547458No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT10810.19312675975817173No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATAGCCCATGAA10230.18276473194505982No Hit
ATACCATATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA10160.1815141423814084No Hit
GTCTTATATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT10040.17937027455800592No Hit
CTCACAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTAAC9220.1647205110980891No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCATGTAGTTAATCA9100.16257664327468663No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA8900.15900353023568253No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA8690.15525176154472822No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT8630.15417982763302698No Hit
GTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCGTTGACATACT8570.15310789372132577No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCTTAACAGTAAATGAAAACAG8460.1511426815498735No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8410.15024940329012249No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC8380.14971343633427187No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC8290.14810553546672003No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCAGAGCA8240.147212257206969No Hit
GCTTACACCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA8150.14560435633941715No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8150.14560435633941715No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT8060.14399645547186532No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7930.14167393199651265No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTGCTGATTTCTTTCCTTCCGCTCG7830.13988737547701058No Hit
CCCTTATCTCCACCTCTAAGGGACACGCATCCTCAGGCCATACAGTGGAA7830.13988737547701058No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAAC7810.13953006417311017No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7710.13774350765360813No Hit
CTCCTAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT7660.1368502293938571No Hit
GCTTTATTCTTCTACTGTACCTAACTTTAATCCTGATTGGCAAACTCCTT7620.1361356067860563No Hit
ATATAAAAGAGAGACGACACGTAGCGCCTCATTTTGCGGGTCACCATATT7610.13595695113410608No Hit
GCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTA7610.13595695113410608No Hit
GGTGTAAGCAATTTGTGGGACCCTTAACAGTAAATGAAAACAGGAGATTA7590.13559963983020568No Hit
ACCCTTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTA7370.13166921548730115No Hit
TTTGCGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACC7360.13149055983535093No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTGCTG7230.1291680363599983No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC7200.12863206940414768No Hit
GGGTAAAGGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7090.1266668572326954No Hit
CCTTTATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG7050.12595223462489458No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7030.1255949233209942No Hit
CCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6970.12452298940929295No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6940.12398702245344233No Hit
GACCTCAAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTT6890.12309374419369132No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6880.1229150885417411No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACGACACGTAGCGCC6790.12130718767418927No Hit
GGTTCATATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6670.1191633198507868No Hit
ACTACATGCTCTGGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTA6600.11791273028713538No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGT6580.11755541898323496No Hit
ATGTAATTGGGAGTTGGGGCTCCTTGCCACAGGAACATATTGTACAAAAA6540.11684079637543412No Hit
GAGTATGTAAATAATGTCTTGTCTGGAAGTAATGATTAACTACATGCTCT6520.11648348507153372No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACAAGACATTATTTACAT6510.1163048294195835No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAAAAGACCCACAATTCG6510.1163048294195835No Hit
CCTCTAAGGGACACGCATCCTCAGGCCATACAGTGGAATTCCACCACTTT6500.11612617376763332No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA6440.11505423985593208No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6430.11487558420398188No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTATTGGGGGC6400.11433961724813126No Hit
ATATGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG6270.1120170937727786No Hit
CTGTTAAGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGC6190.11058784855717695No Hit
CCTATGTCAATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTT6120.10933725899352552No Hit
CATGTATACAAGCGAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6090.1088012920376749No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTAAC6020.10755070247402347No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5980.10683607986622264No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5960.10647876856232225No Hit
GTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCTG5960.10647876856232225No Hit
CCTTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA5950.10630011291037204No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT5950.10630011291037204No Hit
GTTAAGGGTCCCACAAATTGCTTACACCTATCAACAATGTCCTCCTGCAA5950.10630011291037204No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGT5840.10433490073891978No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAGTTGTTCT5810.10379893378306916No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCGAAACAG5790.10344162247916874No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5790.10344162247916874No Hit
GTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATAT5730.10236968856746753No Hit
CTCTTATATAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTC5720.10219103291551732No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGCCCGGTG5700.1018337216116169No Hit
TCCTCAGGCCATACAGTGGAATTCCACCACTTTCCATCAAACTCTTCAAG5670.10129775465576628No Hit
CCCAATTACATAGCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT5650.10094044335186589No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5650.10094044335186589No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA5610.10022582074406507No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5600.10004716509211485No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)