FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434323
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG15520.35733774172677935No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG15310.35250263053073405No Hit
GTGTAAAAGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT13730.3161241748652501No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA13490.3105983334983411No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA12850.29586275651991717No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG12230.28158766632206905No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC12090.27836425885803884No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC10390.2392228825091004No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG10300.2371506919965095No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC10160.23392728453247927No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATCAACTATATGTTCAGG10000.23024339028787333No Hit
CCCTCAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA9500.21873122077347967No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8570.19731858547670741No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8410.19363469123210145No Hit
ATTTATAGGAAGTTTTCGAAAACATTGTTTGATTTTTTGTACAATATGTT8320.1915625007195106No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8260.19018104037778336No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT8220.18926006681663188No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7920.18235276510799567No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7640.1759059501799352No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAA7640.1759059501799352No Hit
ACTATATGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATA7550.17383375966734435No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA7400.17038010881302623No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC7000.1611703732015113No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTCAACTTCAT6850.15771672234719322No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6830.15725623556661747No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6730.15495380166373876No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6620.15242112437057212No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6620.15242112437057212No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6350.14620455283279954No Hit
CTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG6290.1448230924910723No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6260.1441323623202087No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCATT6060.13952749451445123No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6040.13906700773387548No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC6020.13860652095329973No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG5950.13699481722128462No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5780.13308067958639078No Hit
CAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAAGGA5760.13262019280581502No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5740.13215970602523927No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCT5670.13054800229322416No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAA5650.13008751551264844No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5630.12962702873207266No Hit
GGTCTATTTATAGGAAGTTTTCGAAAACATTGTTTGATTTTTTGTACAAT5480.1261733778777546No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5470.12594313448746672No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCAAG5430.1250221609263152No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5380.12387094397487584No Hit
GTATGTAAATAATGCCTGGTTTGGAAGTAATGATCAACTATATGTTCAGG5340.12294997041372435No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5180.11926607616911837No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5150.11857534599825476No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5110.11765437243710328No Hit
GGATATCATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG5090.11719388565652751No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5080.11696364226623963No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5060.1165031554856639No Hit
GGTACGGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTT4970.11443096497307303No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4930.11350999141192156No Hit
ATATGTTCTTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA4890.11258901785077004No Hit
TCGCCAAAGAATCCCAGAGGATTGGGAACAGAAAGATTCGTCCCCATGCC4850.11166804428961856No Hit
GTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCACATTCATTTGAA4850.11166804428961856No Hit
CCCAAGTGTCCTGGCCAAAATTTGCAGTCCCCAACCTCCAATCACTCACT4820.11097731411875494No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4770.10982609716731558No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTTCGAAAAC4570.10522122936155812No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA4550.10476074258098235No Hit
GGGTAACCACATTGTGTAAAAGGAGCGGCAAAACCCAAAAGACCCACAAT4540.10453049919069447No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAT4500.10360952562954298No Hit
CATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAAGGAGC4480.10314903884896724No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4460.1026885520683915No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4460.1026885520683915No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA4450.10245830867810363No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCT4450.10245830867810363No Hit
AGAACATATTGTACAAAAAATCAAACAATGTTTTCGAAAACTTCCTATAA4390.1010768483363764No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)