FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487818

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487818
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645537
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23290.3607848969152814No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATA21730.3366189699428538No Hit
GGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTAACATT21520.33336586438887317No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT21340.33057748819974686No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA20440.31663560725411555No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17590.2724863175929498No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT16170.2504891276565092No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT16040.24847530040880694No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA15930.24677129273767423No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC15230.23592760755773876No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG14950.23159013348576454No Hit
CGTTAATATGGGCCTAAAACTCAGACAATTATTGTGGTTTCACATTTCCT14810.2294213964497775No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA14100.21842280148155724No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13850.2145500567744374No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT13620.2109871316438872No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA12660.19611579196854712No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA11670.18077972292835268No Hit
ACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTA11660.1806248131400679No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC11500.1781462565275112No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT11370.1761324292798089No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11210.1736538726672522No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT11030.17086549647812596No Hit
GGGAAGCAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA10810.16745748113586054No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT10650.16497892452330384No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATCCG10230.15847271341534258No Hit
GTCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA9890.15320578061365964No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG9870.15289596103709005No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT9770.15134686315424212No Hit
CCTCGAAAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT9390.14546029119942003No Hit
GTTCAAATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA9390.14546029119942003No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT9320.14437592268142646No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT9130.14143263670401543No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC8930.13833444093831956No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA8640.13384205707806057No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC8550.13244786898349745No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT8540.13229295919521267No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGACAAAGGGATCAAAC8480.13136350046550394No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACGGCACTCAGGCA8480.13136350046550394No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGACA8200.12702602639352972No Hit
GAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCA8070.12501219914582742No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8040.12454746978097304No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG8000.12392783062783387No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA7920.12268855232155554No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7890.12222382295670117No Hit
GGATAACCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7790.12067472507385324No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT7630.11819616846129657No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG7560.11711179994330302No Hit
ACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTGGC7540.11680198036673342No Hit
CCTGTTTTCATTCACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA7540.11680198036673342No Hit
ACTGCTTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTGGC7510.11633725100187907No Hit
CTCTAAGGCCTCCCGATACATAGCAGAGGCGGTATCGAGGAGATCTCGGA7500.11618234121359425No Hit
GCTATGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCATCTCACCA7490.11602743142530947No Hit
CCCTTACAGTGAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG7450.1154077922721703No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATAT7360.11401360417760717No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG7330.11354887481275279No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT7290.11292923565961362No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT7290.11292923565961362No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT7200.11153504756505049No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA7190.1113801377767657No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAA7190.1113801377767657No Hit
GTTAAGGGAATATCCCCATCTTTTTGTTTTGTGAGGGTTTAAATGTATAC7160.11091540841191132No Hit
ATCCCAATGTTACTAAATATTTGCCCTTAGACAAAGGGATCAAACCGTAT7140.11060558883534174No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7110.11014085947048735No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA7090.10983103989391778No Hit
GTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG7050.10921140074077862No Hit
GCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG7020.10874667137592423No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT6940.10750739306964589No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6930.1073524832813611No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6920.10719757349307632No Hit
TTGACATACTTTCCAATCAATAGGCCTGTTTACAGGAAGTTTCCTAAAAC6920.10719757349307632No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6860.10626811476336755No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTTTG6830.10580338539851318No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6750.10456410709223485No Hit
TTCAAGAACAGTTTGTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA6610.10239537005624774No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTGGATCTTC6590.10208555047967816No Hit
CCCTTAGACAAAGGGATCAAACCGTATTATCCAGAGTATGTAGTTAATCA6560.10162082111482378No Hit
ACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACAT6540.1013110015382542No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6540.1013110015382542No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6540.1013110015382542No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6520.1010011819616846No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6490.10053645259683025No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)