FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487819

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487819
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645537
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23220.35970052839728783No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT21300.3299578490466077No Hit
GGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTAACATT19510.30222899694362987No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA19260.2983562522365101No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATA18690.2895263943042769No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTGTCTCCCAAAAGTAAGA18340.28410455171430915No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16870.2613328128364447No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG15220.23577269776945395No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT14960.23174504327404935No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT13780.21346568825644385No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA13590.2105224022790328No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13510.20928312397275445No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13390.20742420651333696No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT13230.2049456499007803No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT12740.19735507027482546No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC12600.19518633323883836No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12570.194721603873984No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA12490.19348232556770564No Hit
CGTTAATATGGGCCTAAAACTCAGACAATTATTGTGGTTTCACATTTCCT12410.19224304726142732No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT12130.1879055731894531No Hit
ACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTA11030.17086549647812596No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG10980.170090947536702No Hit
GTCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGCATAA10980.170090947536702No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT10250.15878253299191214No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC10030.15537451764964672No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT10020.15521960786136194No Hit
GGGAATTAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA9990.15475487849650754No Hit
GTTCAAATACTGTTTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA9900.15336069040194442No Hit
GGGAAGCAGTAGTCGGCTATGTCAACGTTAATATGGGCCTAAAACTCAGA9620.14902321632997026No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9270.1436013737400025No Hit
CCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA8750.1355460647491933No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATCCG8660.1341518766546302No Hit
GTTTTATCCCAATGTTACTAAATATTTGCCCTTAGACAAAGGGATCAAAC8310.12873003406466246No Hit
GTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGG8160.12640638724039055No Hit
CCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT8140.126096567663821No Hit
ATATTAACGTTGACATAGCCGACTACTGCTTCCCTGGATGCTGGATCTTC8100.12547692851068182No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT7950.12315328168640992No Hit
CCTGTTTTCATTCACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA7920.12268855232155554No Hit
CCCTTACAGTGAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG7850.121604183803562No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7800.12082963486213805No Hit
GCCTTAGAGTCTCCGGAACATTGTTCATCTCACCATACGGCACTCAGGCA7770.12036490549728367No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7760.12020999570899887No Hit
GAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCA7670.11881580761443572No Hit
GTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTC7620.11804125867301177No Hit
CCCCAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT7610.11788634888472697No Hit
CTCTAAGGCCTCCCGATACATAGCAGAGGCGGTATCGAGGAGATCTCGGA7590.1175765293081574No Hit
GGATAACCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT7490.11602743142530947No Hit
CCTCGAAAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT7470.1157176118487399No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT7430.11509797269560072No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG7400.11463324333074634No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG7380.11432342375417677No Hit
GCTTACATCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAA7340.1137037846010376No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT7200.11153504756505049No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT7030.10890158116420902No Hit
CTACATACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATAT6930.1073524832813611No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA6920.10719757349307632No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6890.10673284412822193No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6880.10657793433993715No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6860.10626811476336755No Hit
CTTTCGAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAA6830.10580338539851318No Hit
GTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG6800.1053386560336588No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6690.10363464836252607No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCCAAACAG6690.10363464836252607No Hit
ACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTGGC6640.10286009942110212No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTTCCAATTACATATCCCATGAA6610.10239537005624774No Hit
GCTTTAATCCTAATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAG6590.10208555047967816No Hit
CTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAA6510.10084627217339982No Hit
ACTGCTTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTGGC6500.10069136238511503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)