FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences589155
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42540.7220510731471345No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG17670.2999210734017364No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG16320.2770068997122998No Hit
ATTTACAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT15880.2695385764357427No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT15040.2552808683623155No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG13590.23066934847366144No Hit
ATCCTAACCATACCAAATATTTGCCCCTAGATAAAGGCATCAAACCTTAT12520.21250774414203394No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT12260.20809464402406835No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT11530.19570401676978047No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT11360.19281852823111065No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG11170.18959357045259737No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA11010.18687781653384933No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG10990.1865383472940058No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT10660.180937104836588No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA10270.17431745465963966No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9790.16617019290339555No Hit
GGATAATAAGGTTTGATGCCTTTATCTAGGGGCAAATATTTGGTATGGTT9430.16005974658621244No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGG8980.1524216886897336No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTATAGGAAGTTTCCT8810.1495362001510638No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG8790.14919673091122032No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT8510.14444416155341125No Hit
GTTCTATCCTAACCATACCAAATATTTGCCCCTAGATAAAGGCATCAAAC8100.1374850421366194No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTAAC8080.1371455728967759No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC7970.13527849207763662No Hit
CTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA7660.13001671886006227No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC7660.13001671886006227No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7510.12747069956123602No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7460.12662202646162724No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7320.12424574178272273No Hit
AATAAGGTTTGATGCCTTTATCTAGGGGCAAATATTTGGTATGGTTAGGA7200.12220892634366169No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7090.1203418455245224No Hit
CCTTTATCTAGGGGCAAATATTTGGTATGGTTAGGATAGAACCTAGCAGG7090.1203418455245224No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC7020.11915370318507014No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA7010.1189839685651484No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6900.11711688774600912No Hit
GCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTAATAA6820.1157590107866351No Hit
GTTCCGGAACAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCA6800.1154195415467916No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6700.11372219534757408No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6390.10846042212999975No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG6320.10727227979054749No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC6280.10659334131086047No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6220.10557493359132995No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAGGGGCAAATATTTGGTA6210.10540519897140821No Hit
ATATTAACGTTGACATAGCTTACTACTAATTCCCTGGATGCCGGGTCTTC6160.10455652587179944No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA6140.10421705663195595No Hit
GTAGTAAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACTATT6070.10302891429250369No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6020.10218024119289491No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6000.1018407719530514No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6000.1018407719530514No Hit
CAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGG5920.1004828949936774No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACGAAAAAA5900.10014342575383388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)