FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555745
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTGAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG23090.41547832189223477No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG22640.40738108305067966No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC21530.3874078939081773No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19360.3483612088277897No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19160.3447624360093208No Hit
GGTCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT18530.3334263016311438No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT16300.2932999847052155No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT15750.283403359454426No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT14990.26972802274424423No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG12960.23320047863678484No Hit
GTGGATACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG12610.22690262620446427No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG11950.2150266759035169No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT11790.2121476576487418No Hit
GGCTTAGGCCAACTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGA11000.19793250501578963No Hit
GGGTAACCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAAT10860.19541336404286141No Hit
CTCCAAAAGACACCAAATACTCAAGAACAATATCTCTTCCAAAAGTAAGA10730.1930741617108566No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10700.19253434578808626No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10360.1864164319966891No Hit
GTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG10140.18245778189637335No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9870.1775994385914403No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9590.17256115664558386No Hit
ACGATGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9520.17130158615911975No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC9500.17094170887727286No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9480.17058183159542595No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9450.17004201567265562No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9400.1691423224680384No Hit
GGGTATCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAAT9350.16824262926342118No Hit
CTAACAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA9060.16302440867664128No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA9020.1623046541129475No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT8810.15852594265355513No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCACTATTTCCAATTTTCTT8680.15618674032155036No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA8680.15618674032155036No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8460.15222809022123457No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC8370.15060864245292355No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8290.149169133325536No Hit
CTCCCAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAG8280.14898919468461255No Hit
CAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATG8260.14862931740276564No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC7980.14359103545690918No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGAT7940.1428712808932154No Hit
TCCTCAGGCCATGCAGTGGAACTCCACTGCTGGTGGCTCCAATTCCGGAA7770.13981232399751684No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTGAACG7760.1396323853565934No Hit
GCTTACTACTGCTTCCCTGGATGCCGGGTCTTCCAAATTACCTCCCACCC7650.1376530603064355No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7550.13585367389720104No Hit
AGTCTAGACTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7540.1356737352562776No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGAT7480.13459410341073694No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC7370.13261477836057906No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7200.12955582146488048No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA7190.12937588282395704No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT7180.1291959441830336No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7140.12847618961933982No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA7130.12829625097841635No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7050.1268567418510288No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT7040.12667680321010535No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7030.1264968645691819No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA6990.12577711000548814No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTGAACGAAAAAA6870.1236178463144068No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6790.12217833718701923No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC6710.12073882805963165No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA6710.12073882805963165No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG6650.119659196214091No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA6570.11821968708670343No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6550.11785980980485655No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6500.11696011660023932No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6480.11660023931839242No Hit
GAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGATG6440.11588048475469864No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTCAC6420.11552060747285177No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT6370.11462091426823454No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6360.11444097562731109No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT6310.11354128242269386No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT6290.11318140514084699No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6280.11300146649992353No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6280.11300146649992353No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA6250.11246165057715318No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6210.11174189601345941No Hit
CCTCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG6190.11138201873161252No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTA6170.11102214144976562No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6070.10922275504053117No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG6000.10796318455406706No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAGGTAA5960.10724342999037328No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG5800.10436441173559816No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTGTC5760.10364465717190438No Hit
CAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGATATTGT5740.1032847798900575No Hit
CTTCAAAGACTGTGTATTTAAGGACTGGGAGGAGTTGGGGGAGGAGACTA5710.10274496396728715No Hit
ACTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATA5690.10238508668544027No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5680.10220514804451682No Hit
GTATCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTC5680.10220514804451682No Hit
CTATATGTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATAT5660.10184527076266991No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5650.10166533212174649No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGG5620.10112551619897614No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5600.10076563891712927No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG5600.10076563891712927No Hit
GCCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5570.10022582299435892No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)