FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002487823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences555745
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTTACAGTGAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG22150.3985640896454309No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG21400.3850686915761725No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC20080.36131679097427777No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19260.34656182241855527No Hit
GGTCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT17640.31741176258895715No Hit
GTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTCTTTGACATACT17290.3111139101566366No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16490.29671881888276097No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT14660.26379004759377056No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTT14520.2612709066208423No Hit
GTGGATACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG12510.22510323979522984No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG12400.22312391474507196No Hit
GGGTAACCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAAT11420.2054899279345743No Hit
CTCCAAAAGACACCAAATACTCAAGAACAATATCTCTTCCAAAAGTAAGA11110.1999118300659475No Hit
AAATCGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG10940.19685287317024897No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA10470.18839575704684702No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAACTCAGACAAATATT10380.18677630927853603No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10100.18173802733267957No Hit
GGGTATCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAAT9920.17849913179605756No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9700.17454048169574177No Hit
GGCTTAGGCCAACTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGA9560.17202134072281353No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG9490.1707617702363494No Hit
GTGGTTACCCAGCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAG9290.16716299741788052No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA9280.16698305877695707No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9220.16590342693141638No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT9170.16500373372679916No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA8770.15780618808986135No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8650.15564692439878003No Hit
CTCCCAGTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAG8510.1531277834258518No Hit
GTCTAATAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGAT8510.1531277834258518No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8460.15222809022123457No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA8420.1515083356575408No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCACTATTTCCAATTTTCTT8400.15114845837569388No Hit
CCCTTTACACAATGTGGATACCCAGCATTAATGCCTTTGTATGCATGTAT8390.15096851973477043No Hit
CAATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATG8370.15060864245292355No Hit
ACGATGCAGGACCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8270.1488092560436891No Hit
CCTCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG8100.14575029914799054No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8090.1455703605070671No Hit
CTAACAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATGTA8050.14485060594337332No Hit
TCCTCAGGCCATGCAGTGGAACTCCACTGCTGGTGGCTCCAATTCCGGAA7970.14341109681598577No Hit
GCTTACTACTGCTTCCCTGGATGCCGGGTCTTCCAAATTACCTCCCACCC7850.1412518331249044No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTGAACG7650.1376530603064355No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC7380.1327947170015025No Hit
AGTCTAGACTCTTTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7340.13207496243780872No Hit
GTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGAT7300.13135520787411492No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCA7180.1291959441830336No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7120.1281163123374929No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG7110.1279363736965695No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6990.12577711000548814No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6800.12235827582794266No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA6720.12091876670055511No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGG6690.12037895077778478No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT6610.11893944165039723No Hit
CCTCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGG6560.11803974844578No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6540.1176798711639331No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6530.11749993252300965No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTTGG6380.11480085290915798No Hit
GGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCGTTCAC6280.11300146649992353No Hit
GAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTGCCGATG6250.11246165057715318No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6240.11228171193622975No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6170.11102214144976562No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6120.1101224482451484No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6110.10994250960422497No Hit
GCCTAGCAGCCATGGAAAAGAGGTGTATTTCCGAGAGAGGACAACAGAGT6100.10976257096330153No Hit
GAAATACACCTCTTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC6090.10958263232237807No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6020.10832306183591396No Hit
GTATCCACATTGTGTAAAGGGAGCGGCAAAACCCAAAAGACCCACAATTC5950.10706349134944984No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5940.10688355270852638No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5920.10652367542667951No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5890.10598385950390916No Hit
GTACAAAGATCATTAATCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTA5850.10526410494021538No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCACTAT5800.10436441173559816No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5770.10382459581282782No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5760.10364465717190438No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACGGTACCTGTC5720.10292490260821059No Hit
ATATTTGTCTGAGTTTTAGGCCCATATTAACATTGACATAGCTTACTACT5710.10274496396728715No Hit
GCCTAATCATCTCTTGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5680.10220514804451682No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTATCCACATTGTGTAAAG5670.10202520940359337No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5630.1013054548398996No Hit
ATGTTAATATGGGCCTAAAACTCAGACAAATATTGTGGTTTCACATTTCC5620.10112551619897614No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5560.10004588435343549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)