FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487824

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487824
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290196
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC8820.3039325145763553No Hit
TATTAACATTGACATAGCTTACTACTAAGTCCCTGGATGCTTGATCTTCC8780.3025541358254421No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7710.26568250423851464No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7460.25706763704530733No Hit
ATGTCAATATGGGCATAAAGATCAGACAACTATTGTGGTTTCACATTTCC5940.20468924451060663No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5930.20434464982287834No Hit
CCATCTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGACTGACGATATGGGA5750.19814194544376906No Hit
GTATGTAAATAATGCCGAGTTTGGAAGTAATGATTAACTATGTGTTCAGG5350.18435815793463728No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT5000.172297343864147No Hit
ATGTAAGACCTTGGGCAAGACCTGTCGGGCGTTCACGGTGGTCTCCATGC4920.16954058636232064No Hit
CCTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4850.16712842354822258No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG4850.16712842354822258No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA4640.15989193510592842No Hit
CCCTTTACACAATGTGGGTACCCAGCATTAATGCCTTTGTATGCCTGTAT4620.15920274573047183No Hit
TCCTCAGGCCGTGCAGTGGAATTCCAGCACATTCCACCAAGCTCTGCTAG4600.15851355635501524No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTGTTCG4530.15610139354091718No Hit
CATTTAAACCCTGCCAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT4500.1550676094777323No Hit
GTTTAGGATAAAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG4470.1540338254145474No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4430.15265544666363423No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTTTCT4390.15127706791272105No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT4340.14955409447407958No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4300.1481757157231664No Hit
GAAACGAGGTGTATTTCCGAGACAGGACAACAGAGTTGTCGGTGCCGATA4230.14576355290906834No Hit
CCTCCTTTCCATGGCTGCTAGGTTGTGCTGCCAACTGGATCCTGCGCGGG4180.1440405794704269No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT4110.14162841665632883No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGTTT4110.14162841665632883No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC3990.1374932804035893No Hit
GCTTACTACTAAGTCCCTGGATGCTTGATCTTCCAAATTACTTCCCACCC3940.13577030696494782No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3900.13439192821403464No Hit
CGCTTGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT3810.13129057602448002No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCGAAAGTAAGA3780.13025679196129514No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG3700.12750003445946878No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3670.1264662503962839No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA3660.1261216557085556No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC3620.12474327695764242No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC3590.12370949289445754No Hit
TATTGACATTGACATAGCTTACTACTAAGTCCCTGGATGCTTGATCTTCC3580.12336489820672925No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT3490.12026354601717461No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATACAAGAGAGAAACTACT3440.11854057257853312No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT3420.11785138320307655No Hit
TGTCAGTGCCGATAAGGTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCT3400.11716219382761994No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA3390.11681759913989165No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG3330.11475003101352191No Hit
TTACCCAATGTGGGTACCCAGCATTAATGCCTTTGTATGCCTGTATACAA3330.11475003101352191No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3190.10992570538532577No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG3160.10889192132214089No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTAAGGCA3160.10889192132214089No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCA3110.10716894788349943No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG3050.10510137975712967No Hit
TTGTATGCCTGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTA3030.10441219038167307No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3030.10441219038167307No Hit
CCGAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTT3010.1037230010062165No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT2960.10200002756757501No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2950.10165543287984673No Hit
TCCACAGAGTATGTAAATAATGCCGAGTTTGGAAGTAATGATTAACTATG2940.10131083819211843No Hit
ACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATGTGAGG2920.10062164881666183No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)