FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435387
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24120.553989898641898No Hit
ATCCTAATGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT12760.29307259977904715No Hit
ACTCACAACTGTGCCAACAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12620.28985706968742747No Hit
CATTAGGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCA12530.2877899431999577No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAAAGTCCAA11260.258620491654551No Hit
ATATGGAGGAGGTGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA10810.24828485921720217No Hit
CTTTTATATAAGATACCTGCCTTCCAAAGAGTATGTAAATAATGTCTCGT9180.21084690172191636No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8330.19132404045136855No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC8290.19040531756804865No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8260.18971627540555872No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC8160.187419468197259No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT8100.18604138387227914No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATACCCACATTGTGTGAAA7700.17685415503908017No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCATTCAC7470.17157149845999076No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTTTCGCCAACGTATAAGGCC7410.1701934141350109No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG7280.16720756476422124No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG6750.15503448656023264No Hit
CTGTAAGGGGTCCCACAAATTGTTTACACCTATCAATGATGTTCTCATGC6660.15296736007276285No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC6590.15135959502695304No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTACCCCAA6520.14975182998114323No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTGATTATGCCTGCTAGG6480.14883310709782333No Hit
GGATAATAAGGTTTAATTCCTTTATCTAAGGGCAAATATTTAGTAACATT6330.1453878962853737No Hit
CATTTAAACCCTCAGAAAACAAAAAGATGGGGCTACTCCCTTAACTTCAT6130.1407942818687742No Hit
ATATAAGATACCTGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA6130.1407942818687742No Hit
ATGTTAATATGGGCATAAAACTCAGACAACTATTGTGGTTTCACATTTCC6120.14056460114794422No Hit
TACCCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCG5910.1357413060105148No Hit
GTATAAGGCCTTTCTAAGCAAACAATATCTGAACCTTTACCCCGTTGCTC5880.13505226384802485No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5790.1329851373605551No Hit
GCCTTATACGTTGGCGAAAAAGTAAAAGCCTGTTTAGCTTGTATACATGC5770.13252577591889517No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGAGGCTGGGTCTTCC5370.12333854708569618No Hit
GTTAATATGGGCATAAAACTCAGACAACTATTGTGGTTTCACATTTCCTG5360.1231088663648662No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5230.12012301699407654No Hit
GTTTACACCTATCAATGATGTTCTCATGCAAATGAATGTCAGGAAAGGAA5230.12012301699407654No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGC5200.1194339748315866No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGGACGACAGAAT5180.11897461338992667No Hit
CTCTTATGCAAGACCTTGGGCAGGTTCCGATGGGCGTTCACGGTGGTCTC5100.11713716762328688No Hit
GTTCTATCCTAATGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAAC5040.11575908329830703No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT4930.11323259536917732No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTGTTTGATTTTCAAC4910.11277323392751736No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4820.1107061074400476No Hit
GTATAAAGAATTTGGAGCTTCTTTGGAGTTACTCTCTTTTTTGCCTTCTG4820.1107061074400476No Hit
AACATATTGTGTTGAAAATCAAACAATGTTTTAGGAAACTTCCTGTTAAC4820.1107061074400476No Hit
CAATATGTTCTTGGGGTAAGGTGCCCCAACTTCCAATTACATATCCCATG4810.11047642671921762No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG4800.11024674599838764No Hit
CATATAAAGGCATTAAAGCAGGATACCCACATTGTGTGAAAGGAGCAGCA4800.11024674599838764No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4740.10886866167340781No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATCA4710.10817961951091787No Hit
GTACACCATCTTGAGGCCCTTCATACCGCTGTTACCAATTTTCTTGTGTC4690.10772025806925793No Hit
TCATTGATAGGTGTAAACAATTTGTGGGACCCCTTACAGTGAATGAAAAC4680.10749057734842796No Hit
GCTTTATTCTTCTACGGTACCTATCTTTAATCCTCAATGGCAAACTCCTT4580.1051937701401282No Hit
GGGTAAAGGTTCAGATATTGTTTGCTTAGAAAGGCCTTATACGTTGGCGA4510.1035860050943184No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG4490.10312664365265845No Hit
AGTTAAGGGAGTAGCCCCATCTTTTTGTTTTCTGAGGGTTTAAATGTATA4480.10289696293182846No Hit
TCCTCAGGCAATGCAGTGGAACTCCACCACGTTCCACCAAACTCTTCAAG4420.1015188786068486No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4400.10105951716518867No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4370.10037047500269876No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)