FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487827

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487827
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435387
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN23970.5505446878294483No Hit
ATATGGAGGAGGTGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA9900.2273839136216745No Hit
ACTCACAACTGTGCCAACAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9890.22715423290084452No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATCGCTGAAAGTCCAA9450.21704828118432568No Hit
ATCCTAATGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAACCTTAT9260.21268434748855616No Hit
CATTAGGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCA9060.20809073307195666No Hit
CTTTTATATAAGATACCTGCCTTCCAAAGAGTATGTAAATAATGTCTCGT8390.19270212477634838No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC7980.18328521522231947No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7940.18236649233899954No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATACCCACATTGTGTGAAA7880.18098840801401972No Hit
CTGTAAGGGGTCCCACAAATTGTTTACACCTATCAATGATGTTCTCATGC7120.16353267323094167No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTGTTTTCATTCAC7000.16077650458098197No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC6890.15825001665185226No Hit
TCTTTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6440.1479143842145034No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAACACCCGTG6430.14768470349367344No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGCACCTTACCCCAA6280.14423949268122382No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6240.14332076979790392No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG6220.142861408356244No Hit
GTTGTGAGTATGCCCTGAGCTTGAGGGCTCCACCCCAACAGTCCCCCATG6220.142861408356244No Hit
GGATAATAAGGTTTAATTCCTTTATCTAAGGGCAAATATTTAGTAACATT5910.1357413060105148No Hit
CCCTTACAGTGAATGAAAACAGGAGACTAAAATTGATTATGCCTGCTAGG5870.1348225831271949No Hit
GTATAAGGCCTTTCTAAGCAAACAATATCTGAACCTTTACCCCGTTGCTC5770.13252577591889517No Hit
ATGTTAATATGGGCATAAAACTCAGACAACTATTGTGGTTTCACATTTCC5610.12885088438561557No Hit
GTTTACACCTATCAATGATGTTCTCATGCAAATGAATGTCAGGAAAGGAA5330.12241982420237627No Hit
ATATAAGATACCTGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA5320.1221901434815463No Hit
CCTCCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC5300.12173078203988635No Hit
GCCTTATACGTTGGCGAAAAAGTAAAAGCCTGTTTAGCTTGTATACATGC5270.12104173987739643No Hit
CATTTAAACCCTCAGAAAACAAAAAGATGGGGCTACTCCCTTAACTTCAT4820.1107061074400476No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA4820.1107061074400476No Hit
GGGTAAAGGTTCAGATATTGTTTGCTTAGAAAGGCCTTATACGTTGGCGA4680.10749057734842796No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAAAGGACGACAGAAT4680.10749057734842796No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTTTCGCCAACGTATAAGGCC4630.10634217374427808No Hit
GTTCTATCCTAATGTTACTAAATATTTGCCCTTAGATAAAGGAATTAAAC4520.10381568581514836No Hit
TACCCACATTGTGTGAAAGGAGCAGCAAATCCCAAAAGACCCACAATTCG4440.10197824004850857No Hit
GTACACCATCTTGAGGCCCTTCATACCGCTGTTACCAATTTTCTTGTGTC4380.10060015572352873No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)