FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487830

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487830
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535022
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54251.0139769953385094No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG46580.8706184044768253No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT27990.5231560571340992No Hit
ATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAACCTTAT20800.38876905996388933No Hit
GTATAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC18550.3467147145351032No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17230.3220428318835487No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAA15190.28391355869478263No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA13790.2577464104279824No Hit
CTCCAAAGGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12690.23718650821835363No Hit
TAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGA12010.22447675048876495No Hit
ATTAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAG11680.21830877982587632No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11540.21569206499919633No Hit
CTCTAACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10080.18840346752096176No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC10020.18728201830952745No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9450.17662825080090164No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9410.17588061799327878No Hit
ACCAAATACTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA9340.17457226057993877No Hit
ATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTAAAATTAATTA9200.17195554575325875No Hit
GAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTAAAATT8970.1676566571094273No Hit
CTAACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT8750.16354467666750153No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT8470.15831124701414148No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8460.15812433881223575No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8400.15700288960080144No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAGTATTTGGTAAGGTT8140.15214327635125285No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT8120.1517694599474414No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7590.14186332524643847No Hit
GTTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7590.14186332524643847No Hit
GTTTTATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAAC7530.14074187603500418No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7320.13681680379498412No Hit
CCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCA7000.13083574133400122No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG6940.1297142921225669No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT6850.12803211830541547No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT6790.1269106690939812No Hit
CAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTAAAATTAAT6660.12448086246920687No Hit
GATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTA6650.12429395426730115No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC6620.123733229661584No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6600.12335941325777257No Hit
GTATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATC6580.12298559685396115No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAGTATTTGGTA6570.12279868865205544No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6530.12205105584443257No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAGT6490.1213034230368097No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6460.12074269843109255No Hit
ATATTATACTGAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGG6310.11793907540250681No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6240.11663071798916681No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT6230.11644380978726108No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6170.11532236057582679No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6100.11401400316248679No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA5980.11177110473961818No Hit
CATTTACAGGAGGACATTATTAATAGATGTGAACAATATGTGGGCCCTCT5970.11158419653771245No Hit
CATTATTAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAA5830.10896748171103243No Hit
ATCCTAAACTTACCAAATACTTGCCCTTGGATAAAGGCATTAAACCTTAT5750.10747221609578672No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACATAC5710.10672458328816385No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5690.10635076688435242No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC5580.10429477666338954No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5500.1027995110481438No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5440.10167806183670952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)