FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences535022
Sequences flagged as poor quality0
Sequence length35-151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53961.0085566574832439No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG48580.9080000448579685No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT29310.5478279397856537No Hit
GTATAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC20660.3861523451372093No Hit
ATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAACCTTAT19540.36521862652376913No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT18770.35082669497702895No Hit
TTATTAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAA14840.2773717716280826No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCA14200.2654096467061167No Hit
CTCCAAAGGATACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12530.2341959769878622No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11560.21606588140300775No Hit
TAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGA10740.20073940884673902No Hit
ATTAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAG10150.18971182493430178No Hit
ATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTAAAATTAATTA10090.18859037572286746No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA10000.18690820190571603No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC9820.18354385427141315No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC9650.18036641483901597No Hit
CTCTAACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG9580.17905805742567596No Hit
GAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTAAAATT9500.17756279181043022No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAGTATTTGGTAAGGTT8980.167843565311333No Hit
CTAACAACTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT8710.16279704385987867No Hit
GTTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8700.16261013565797294No Hit
CCTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGA8450.15793743061033005No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA8350.1560683485912729No Hit
ACCAAATACTTGCCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACA7950.14859202051504425No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC7900.14765747950551564No Hit
ATATTATACTGAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGG7810.14597530568836423No Hit
GTATTTGGTAAGGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATC7630.14261095805406132No Hit
GTTTTATCCTAACCTTACCAAATACTTGCCCTTGGATAAAGGCATTAAAC7630.14261095805406132No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT7420.1386858858140413No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGGCATACT7370.1377513448045127No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7290.13625607918926697No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGAT7190.13438699717020983No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7160.13382627256449267No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAGT7100.13270482335305836No Hit
CCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAATCCTAAT7040.13158337414162408No Hit
ATGTTAATATGGGCCTGAAAATCAGACAACTATTGTGGTTTCACATTTCC7020.13120955773781265No Hit
GATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTA6980.1304619249301898No Hit
CAATATGTGGGCCCTCTAACAACTAATGAAAAAAGGAGATTAAAATTAAT6880.12859284291113263No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAGTATTTGGTA6800.1270975772958869No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6760.12634994448826403No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATACCTCTATTACCAATTTTCTT6720.12560231168064118No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA6630.12392013786348972No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6390.11943434101775255No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6330.11831289180631824No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT6210.11606999338344966No Hit
CCCTTGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCA6210.11606999338344966No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6010.11233182934533532No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGATTCAAATGTATA5850.10934129811484387No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5810.108593665307221No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5800.10840675710531529No Hit
CATTTACAGGAGGACATTATTAATAGATGTGAACAATATGTGGGCCCTCT5750.10747221609578672No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAA5590.10448168486529527No Hit
CATTATTAATAGATGTGAACAATATGTGGGCCCTCTAACAACTAATGAAA5500.1027995110481438No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)