FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378571
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG15100.3988683760774069No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT13090.34577397634789775No Hit
AGTCTATACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12800.33811359031727206No Hit
GTATACATGCATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAA12030.3177739446497487No Hit
CATGTATACAGTCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10520.277887107042008No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10350.2733965359206067No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGCACCCGTG10110.2670569061021579No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA8890.23483045452504284No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8800.23245309334312456No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTCGGAAAGGAGGGAGTTTG8630.22796252222172328No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8230.21739647252430852No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT8100.21396250637264871No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8070.2131700526453426No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8030.21211344767560117No Hit
CTATTATGGTTTCACATTTCCTGTCTTACCTTTGGAAGAAATACCGTTCT7580.20022664176600952No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7480.19758512934165587No Hit
ATTGACAACAGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT7320.19335870946268996No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC7250.19150965076564236No Hit
GTGTAAAAGGAGCAGCAAAGCCCAAAAGACCCACTATTCTTTGACATACT6980.18437756721988743No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT6930.18305681100771057No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCGAAC6830.1804152985833569No Hit
GTATAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6620.17486812249221415No Hit
CTCCAAAAGACACCAAATACTCAAGAACGGTATTTCTTCCAAAGGTAAGA6520.17222661006786047No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6250.1650945265221055No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6070.16033980415826884No Hit
ATGATTAACTGCGTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG5990.1582265942187859No Hit
GAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCGTGTTCA5970.15769829173391517No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT5930.1566416867641737No Hit
GTCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5890.15558508179443223No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5870.1550567793095615No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA5780.15267941812764318No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT5770.1524152668852078No Hit
GATTAACTGCGTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC5750.15188696440033705No Hit
GTCTTATATAAGAGGACTCTTGGACTCCCAGCAATGTCAACGACCGACCT5710.15083035943059558No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5700.15056620818816022No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5670.1497737544608541No Hit
TCCTCAGGCCATGCAATGGAATTCCACAACTCTTTCTGCTGGTGGCTCCA5530.14607563706675894No Hit
CCATAATAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGTTGA5510.1455473345818882No Hit
CATTTGAACCCTCATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT5240.13841525103613325No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5140.13577373861177958No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTATGGTTTCACATTTCC5080.13418883115716734No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5020.13260392370255514No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4980.13154731873281367No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCGAACATTCATTTACAGG4970.1312831674903783No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4880.12890580630845996No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATA4800.12679259636897702No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT4760.12573599139923555No Hit
GTTTTAATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACA4740.1252076889143648No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4700.12415108394462333No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4690.12388693270218797No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4650.12283032773244648No Hit
CTATACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4610.12177372276270503No Hit
GGAACATATTGTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAA4560.12045296655052817No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4510.11913221033835132No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA4500.11886805909591595No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC4420.116754849156433No Hit
TCCACAGAGTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCG4400.11622654667156228No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4220.11147182430772562No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTATGGTTTCACATTTCCTG4190.11067937058041953No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGTCTTTATTC4190.11067937058041953No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4170.1101510680955488No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4020.10618879945901827No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4020.10618879945901827No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTATACAACATCTTG4010.10592464821658287No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTATACAACATCTTGAG3990.10539634573171214No Hit
CTCATAAGGTGGGAAACTTTACTGGTCTTTATTCTTCTACTGTACCTGTC3990.10539634573171214No Hit
CCTTTTACACAATGTGGCTATCCCGCCTTGATGCCTTTATATGCATGTAT3980.10513219448927678No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA3970.10486804324684142No Hit
ATACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC3950.10433974076197068No Hit
AGTTAAGGGAGTAGCCCCAGCGTTTGGTTTTATGAGGGTTCAAATGTATA3950.10433974076197068No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3940.10407558951953531No Hit
GTTTTGAAGTAATGATTAACTGCGTGTTCAGGATAATAAGGTTTAATGCC3930.10381143827709993No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGAGTTTTAATACAATATGTT3880.1024906820649231No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA3880.1024906820649231No Hit
CATATAAAGGCATCAAGGCGGGATAGCCACATTGTGTAAAAGGAGCAGCA3870.10222653082248773No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG3870.10222653082248773No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAGGAACATATTGTATTAAAA3840.10143407709518161No Hit
GTCCCGTGCTGGTAGTCGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3830.10116992585274624No Hit
TATTAACATTGACATAGTTGACTATTGCTTCCCTGGATGCTGGGTCTGGC3790.10011332088300476No Hit
ATGTGGTATTGGGGGCCAAGTCTATACAACATCTTGAGTCCCTTTTTACC3790.10011332088300476No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)