FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487834

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487834
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences311525
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10230.32838455982665915No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6930.22245405665676912No Hit
ACCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5900.18939089960677313No Hit
ACTTAAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG5300.17013080812133857No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5280.1694888050718241No Hit
ATATAAAAGAGAAACCGCACGAAGCGCTTCATTTTGTGGGTCACCATATT5170.16595778829949442No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5110.16403177915095096No Hit
GGTTCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG5080.16306877457667923No Hit
ATATCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5050.1621057700024075No Hit
ATGTAAGACCTTGGGCAAGGCCTGGTGGGCGTTCACGGTGGTTTCCATGC5050.1621057700024075No Hit
GGTTTAAGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4940.15857475323007786No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCCGCAATGTCAACGACCGACCT4910.1576117486558061No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4900.15729074713104887No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACCGCA4860.15600674103201992No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGATC4660.14958671053687506No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGGCCTGGTGGGCGTTC4630.14862370596260333No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4540.14573469223978813No Hit
TACTTAAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGT4500.14445068614075918No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4410.14156167241794398No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA4380.14059866784367225No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT4380.14059866784367225No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT4230.13578364497231363No Hit
CTGTAAGAGGGCCCACAAATTGTTGACATCTATTAATAATGTCTTCCTGT4110.1319316266752267No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG4100.13161062515046948No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4080.13096862210095497No Hit
GTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAATCTAAGCAG4070.13064762057619772No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4060.1303266190514405No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCGGAGAGTCCAA4050.13000561752668324No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG3950.12679560227911083No Hit
CACATTTCCTGTCTTACTTTTGGAAGACAAACTGTTCTTGAGTATTTGGT3870.12422759008105287No Hit
GTTTTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3860.12390658855629565No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3800.12198057940775218No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3770.12101757483348047No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTGTCTTCCAAAAGTAAGA3730.1197335687344515No Hit
AGTCTAGCCTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3690.11844956263542253No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT3670.11780755958590804No Hit
CTCCCACCTGATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCC3660.11748655806115078No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACCGCAC3600.11556054891260732No Hit
GGAAACGTGAAACCACAATAGTTGTCTGAATTTTAGGCCCATATTAGCAT3560.11427654281357835No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA3500.1123505336650349No Hit
GGGTTCACCCCGCCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA3490.11202953214027767No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG3460.11106652756600593No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT3430.1101035229917342No Hit
AGGTAGGAGCGGGAGCATTCGGGCCAGGGTTCACCCCGCCACACGGCGGT3410.10946151994221973No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3400.10914051841746249No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC3320.10657250621940455No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3290.1056095016451328No Hit
ACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGA3270.10496749859561832No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA3240.10400449402134661No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3170.1017574833480459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)