FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487836

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487836
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462157
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGG33600.727025664438708No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT29380.6357147030121799No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG17350.375413549940821No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15210.3291089391700223No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC14160.30638938715631264No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT13920.3011963466960362No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA13390.2897283823462589No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12790.2767457811955677No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG12310.26635970027501477No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11630.25164608563756474No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT11490.24861681203573677No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT11280.24407290163299486No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC11240.24320739488961543No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCG11120.24061087465947717No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA11050.2390962378585632No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCA10500.2271955201370963No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT10380.22459899990695803No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10180.22027146619006097No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA9770.21140002207042197No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9680.20945263189781826No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9650.20880350184028373No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT9550.20663973498183516No Hit
TTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9430.2040432147516969No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC9020.19517177063205796No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8650.18716583325579836No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG8440.18262192285305642No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8280.17915989587953876No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC8150.17634699896355568No Hit
ATGATTAACTGCGTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGG7920.17137033518912403No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG7830.16942294501652036No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA7570.16379715118455415No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC7520.1627152677553299No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7420.16055150089688136No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7410.1603351242110365No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7340.15882048741012253No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC7320.15838773403843281No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG7240.15665672055167398No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC7220.1562239671799843No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC7190.15557483712244974No Hit
TTATTATCCTGAACACGCAGTTAATCATTACTTCAAAACTAGGCATTATT7170.15514208375076002No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTACTAG7160.15492570706491518No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7000.15146368009139752No Hit
GTTTTGAAGTAATGATTAACTGCGTGTTCAGGATAATAAGGTTTAATGCC7000.15146368009139752No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA6990.15124730340555265No Hit
CCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG6950.15038179666217324No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6850.1482180298037247No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6800.14713614637450045No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA6670.14432324945851735No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6650.14389049608682764No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6620.14324136602929308No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6600.14280861265760336No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6430.13913020899824086No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6370.13783194888317174No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC6340.13718281882563718No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6300.13631731208225778No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG6200.13415354522380923No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6140.13285528510874012No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAAT6070.13134064830782613No Hit
GATTAACTGCGTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC6070.13134064830782613No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACACGCAGTTAATCATTACT6060.1311242716219813No Hit
CTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCGAAAAC5980.12939325813522246No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC5980.12939325813522246No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5960.12896050476353274No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5900.12766224464846362No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT5890.12744586796261875No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATATGTT5790.12528210110417023No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAAT5790.12528210110417023No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5770.12484934773248052No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5710.12355108761741139No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5700.12333471093156656No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA5680.12290195755987683No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5610.12138732075896287No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5560.12030543732973858No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCGTGTTCAGGAT5440.11770891709960035No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC5430.11749254041375551No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT5410.11705978704206578No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5360.11597790361284152No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5290.11446326681192755No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG5240.11338138338270329No Hit
GAATATGGTGACCCACAAAATGAAGCGCTGCGTGTAGTTTCTCTTTTATA5240.11338138338270329No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA5220.11294863001101359No Hit
GCTAGGTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCAT5120.11078486315256504No Hit
AACATGGAGAGCACAACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5020.1086210962941165No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4990.10797196623658194No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC4960.10732283617904738No Hit
CAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATG4950.10710645949320254No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4890.10580819937813342No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG4860.10515906932059885No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4790.10364443251968487No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4770.10321167914799517No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4760.10299530246215031No Hit
ATACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4730.10234617240461574No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4720.10212979571877089No Hit
CTTTACCACAGGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAA4690.10148066566123633No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA4650.10061515891785693No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG4640.10039878223201207No Hit
ATACAGCACTCAGGCAAGCTATTCTCTGTTGGGGTGAGTTGATGAATCTG4640.10039878223201207No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4630.10018240554616721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)