FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487843

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487843
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences376000
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG23970.6375No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG20570.5470744680851064No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAAGTT14150.3763297872340426No Hit
GTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC12160.32340425531914896No Hit
CCTTTATACCGCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTGA11480.3053191489361702No Hit
GTTTGGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTTTAATGCC10680.28404255319148936No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGTTT9700.2579787234042553No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCCTGT8570.2279255319148936No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8550.2273936170212766No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG7710.20505319148936169No Hit
CTTCCACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCT7210.19175531914893618No Hit
CTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCCTAGT7200.19148936170212766No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC7190.19122340425531914No Hit
GTGTCAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC6930.18430851063829787No Hit
CTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTT6440.17127659574468085No Hit
CTGTCCAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTGTG6390.16994680851063831No Hit
ATTCGTTGACATACTTTCCAATCAATAGGTCTATTTACAGGCAGTTTCCG6380.1696808510638298No Hit
CTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCT6370.16941489361702128No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAA6050.16090425531914893No Hit
GGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG6020.1601063829787234No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5990.15930851063829787No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5980.15904255319148936No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAGTACCCAAG5960.15851063829787235No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5850.1555851063829787No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTATCTCTTCCAAAAGTAAGA5830.1550531914893617No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA5810.1545212765957447No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGGTCAGG5690.15132978723404256No Hit
ATCCTAACTTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5610.14920212765957447No Hit
CATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCT5570.14813829787234042No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA5500.14627659574468085No Hit
CCTTTATCCAAGGGCAAATATTTGGTAAAGTTAGGATAAAACCTAGCAGG5320.14148936170212767No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5310.14122340425531915No Hit
GTATGTAAATAATGCTTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG5250.1396276595744681No Hit
GGAACATATTGTACAAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5240.13936170212765958No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5230.13909574468085106No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA5090.13537234042553192No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATA5070.1348404255319149No Hit
TCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTG4960.13191489361702127No Hit
ATTTACAGGAGGACATTATTGATAGATGTCAACAATATGTGGGCCCTCTT4950.13164893617021275No Hit
GTTTTATCCTAACTTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4950.13164893617021275No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4890.1300531914893617No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAAGTTAGGATAAAAC4890.1300531914893617No Hit
CAGTAGAAGAATAAAGCCCAGTAAAATTTCCCACCTTATGAGTCCAAGGA4880.1297872340425532No Hit
GTTAACATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCCTG4870.12952127659574467No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4760.12659574468085105No Hit
CTCAAGAACAGTATCTCTTCCAAAAGTAAGACAGGAAACGTGAAACCACA4660.12393617021276597No Hit
GTCCAACATCTTGAGTCCCTTTATACCGCTATTACCAATTTTCTTGTGTC4640.12340425531914895No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC4600.12234042553191489No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAAT4570.12154255319148936No Hit
GGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAAT4560.12127659574468085No Hit
ATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAAGTTAG4540.12074468085106382No Hit
CTCATAAGGTGGGAAATTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4530.12047872340425533No Hit
ACATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAA4490.11941489361702126No Hit
GTTTTGAAGTAATGATTAACTGCATGGTCAGGATAATAAGGTTTAATGCC4490.11941489361702126No Hit
ATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG4480.11914893617021277No Hit
ATATAAGAGAGAATCTACACGCAGCGCTTCATTTTGTGGGTCACCATATT4480.11914893617021277No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCAATCAGGCA4460.11861702127659575No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC4450.11835106382978723No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4440.11808510638297873No Hit
GAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACTT4430.11781914893617021No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG4420.1175531914893617No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGGCTTTATTC4420.1175531914893617No Hit
GATTAACTGCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGC4420.1175531914893617No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGCATGTTCAGG4400.11702127659574468No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG4370.11622340425531914No Hit
CTTTAGGACATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA4360.11595744680851063No Hit
CAATAGGTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGT4320.1148936170212766No Hit
CTGTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC4300.11436170212765957No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT4250.11303191489361701No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC4230.11249999999999999No Hit
ATCTAGGGAATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAAT4220.1122340425531915No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA4200.11170212765957448No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGACGTTGACATTGCTGAGAGTCCAA4100.10904255319148935No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTT4100.10904255319148935No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4090.10877659574468085No Hit
ATGCTTAGTTTTGAAGTAATGATTAACTGCATGTTCAGGATAATAAGGTT4080.10851063829787233No Hit
ATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTTGTACAATATGTT4070.10824468085106384No Hit
AGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA4060.10797872340425532No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCT4050.10771276595744682No Hit
GCATGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTT4040.1074468085106383No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCC4040.1074468085106383No Hit
CATTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA4040.1074468085106383No Hit
GGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4040.1074468085106383No Hit
CCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTA4020.10691489361702128No Hit
GTGTAGATTCTCTCTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAA4010.10664893617021276No Hit
GTAAGGGGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAA4000.10638297872340426No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC3980.10585106382978723No Hit
CTATATAAGAGAGAATCTACACGCAGCGCTTCATTTTGTGGGTCACCATA3980.10585106382978723No Hit
ATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTA3960.10531914893617021No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC3930.10452127659574467No Hit
CCTCCCCCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3920.10425531914893618No Hit
GATTAAAATTAATTATGCCTGCTAGGTTTTATCCTAACTTTACCAAATAT3910.10398936170212766No Hit
CTTATATAGAATGCCAGCTTTCCACAGAGTATGTAAATAATGCTTAGTTT3900.10372340425531913No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAATTTCCCAC3900.10372340425531913No Hit
GTACCCAAGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTC3880.10319148936170212No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3870.10292553191489362No Hit
ACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAA3830.10186170212765958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)