FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478706
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG38610.8065493225487043No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT29790.6223026241576249No Hit
CTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG21660.4524697831236709No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA19570.4088104180854219No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17190.35909305502751165No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA14740.3079134165855452No Hit
ATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATATGTT13680.2857703893412658No Hit
GTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA13460.2811746667056607No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12910.2696853601166478No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12840.26822308473259165No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12610.26341846561354987No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12110.25297364144171997No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11970.2500490906736076No Hit
ATCCTACCCGTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT11580.24190212781958026No Hit
CATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT11160.23312847551524318No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA10840.22644378804527204No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTTCGAAAAC10600.2214302724427937No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA10500.21934130760842774No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10270.21453668848938598No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCATTAAC10220.21349220607220298No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATA10150.21202993068814682No Hit
GGGTAGGATAGAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCA9970.20826979398628803No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9850.20576303618504888No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9790.20450965728442927No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9320.19469152256290917No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGACCGCCGTG9290.1940648331125994No Hit
ATTGACAACAGTGCCAGCGGCACCTCCTCCTGCCTCCACCAATCGGCAGT8990.18779793860950145No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8830.18445559487451588No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC8780.18341111245733288No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC8520.17797980388798135No Hit
GTTCTATCCTACCCGTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC8470.17693532147079835No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG8350.17442856366955917No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8310.17359297773581278No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC8270.1727573918020664No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTTT8240.17213070235175662No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACTTGGGTGGGAGGTAA8230.17192180586832No Hit
GTCCCGTGCTGGTCGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8190.17108621993457362No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC8180.17087732345113701No Hit
CAATAGGTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGT8110.16941504806708085No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7980.16669939378240506No Hit
ATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAAGAAGT7700.16085029224618033No Hit
GGTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT7670.16022360279587053No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA7660.16001470631243395No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTG7640.15959691334556075No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAAGAACAT7570.15813463796150456No Hit
ATGTTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCATATTTCC7520.15709015554432157No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7490.1564634660940118No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7470.15604567312713857No Hit
CTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTT7420.15500119070995558No Hit
TTTCCGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7190.15019657159091385No Hit
TCTTTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCCATTCG7160.14956988214060404No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7100.14831650323998447No Hit
GGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7090.14810760675654785No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAAGAACATATTGTACAAAAA7010.14643643488905506No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATATT6960.1453919524718721No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6890.1439296770878159No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6850.1430940911540695No Hit
ATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT6650.13891616148533756No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTAAAGG6570.1372449896178448No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6480.1353649212669154No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA6410.1339026458828592No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6380.13327595643254941No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACTGTACCTGTC6300.13160478456505664No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT6260.13076919863131023No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6190.12930692324725407No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC6120.12784464786319788No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6120.12784464786319788No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6080.12700906192945147No Hit
GGCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6060.12659126896257827No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5990.1251289935785221No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5970.12471120061164892No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATGTAA5960.1245023041282123No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG5850.12220444281040974No Hit
AGAACATATTGTACAAAAAATCAAGCAATGTTTTCGAAAACTACCTGTAA5830.12178664984353654No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTACGGGTAGGATAGAAC5790.12095106390979013No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAAT5760.12032437445948034No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG5750.12011547797604374No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTACGGGT5720.11948878852573395No Hit
TATCCAAGGGCAAATATTTGGTACGGGTAGGATAGAACCTAGCAGGCATA5700.11907099555886076No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG5690.11886209907542417No Hit
ACCCAGCATCCCGGGAAGCAGTAGTCAGCTATGTCAATGTTAATATGGGC5690.11886209907542417No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5660.11823540962511436No Hit
AGTCTAGCCTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5520.11531085885700201No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA5490.1146841694066922No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5480.1144752729232556No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA5440.11363968698950921No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC5380.11238630808888964No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5360.11196851512201644No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA5330.11134182567170664No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5320.11113292918827004No Hit
ACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG5300.11071513622139685No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5290.11050623973796025No Hit
TTCCTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC5280.11029734325452366No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGGAT5270.11008844677108706No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5250.10967065380421387No Hit
CTCTAAGGCCTCCCGATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA5230.10925286083734068No Hit
CCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5230.10925286083734068No Hit
GTACAGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5230.10925286083734068No Hit
GTTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCATATTTCCTG5210.10883506787046748No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5150.10758168896984788No Hit
GTTTTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAAT5140.10737279248641128No Hit
ATCCTGAAAATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACAT5130.10716389600297468No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCAAGCTTACAGACCACCAAATGCC5090.1063283100692283No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5090.1063283100692283No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA5080.1061194135857917No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5050.1054927241354819No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG5010.10465713820173551No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT4940.10319486281767934No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTCTATCCTACCCG4930.10298596633424274No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACTG4920.10277706985080615No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4900.10235927688393293No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4820.10068810501644015No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4810.10047920853300356No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4810.10047920853300356No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT4810.10047920853300356No Hit
GTATAAAGAATTTGGAGCTTCTTTGGAGTTACTCTCTTTTTTACCTTCTG4800.10027031204956696No Hit
TAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGA4800.10027031204956696No Hit
TAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4800.10027031204956696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)