FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478706
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGG37700.7875397425559738No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT28890.6035019406483312No Hit
CTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGG19920.4161217950057029No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA18380.3839517365564668No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT17830.37246242996745393No Hit
CCCTTAACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA14620.305406658784306No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT14140.2953796275793493No Hit
GTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATA13940.29120169791061734No Hit
ATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGTACAATATGTT13760.28744156120875863No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA12630.2638362585804231No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT12480.2607028113288741No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12330.2575693640773251No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA11600.24231992078645345No Hit
CATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCT11040.23062171771400403No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA10940.228532752879638No Hit
ATCCTACCCGTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCGTAT10800.22560820211152566No Hit
GAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCA10760.22477261617777927No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10490.21913241112499113No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT10250.2141188955225128No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC9510.19866055574820451No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATAAA9420.19678048739727513No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9390.19615379794696536No Hit
GGGTAGGATAGAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCA9330.19490041904634578No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATA9330.19490041904634578No Hit
GTTCTATCCTACCCGTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC9310.1944826260794726No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAGTCTCCTTTTTTCATTAAC9130.1907224893776138No Hit
TTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTTCGAAAAC9090.1898869034438674No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTG9070.18946911047699422No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCAACCCAAAAGACCGCCGTG8950.18696235267575506No Hit
ATTGACAACAGTGCCAGCGGCACCTCCTCCTGCCTCCACCAATCGGCAGT8850.1848733878413891No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC8540.17839759685485457No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTTT8450.17651752850392516No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8320.1738018742192494No Hit
GTCCCGTGCTGGTCGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8260.1725484953186298No Hit
GCACGGGACCATGCAAGACCTGCACAATTCCTGCTCAAGGAACCTCTATG8250.1723395988351932No Hit
CTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAA7910.16523711839834887No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTGGCCACTTGGGTGGGAGGTAA7880.1646104289480391No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7850.1639837394977293No Hit
ATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAATGGAAGGAAAGAAGT7830.1635659465308561No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGCGGGTCACCATATT7720.16126808521305352No Hit
GGTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGTACAAT7610.15897022389525095No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCCTATCAACACTTCCGGA7490.1564634660940118No Hit
GTTTTGAAGTAATGATTAACTGCATTTTCAGGATAATACGGTTTAATGCC7440.15541898367682877No Hit
CAATAGGTCTATTTACAGGTAGTTTTCGAAAACATTGCTTGATTTTTTGT7410.154792294226519No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTAAAGG7250.15144995049153342No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7240.15124105400809684No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA7210.15061436455778704No Hit
TTTCCGGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTA7050.14727202082280147No Hit
TTCCCCCACTGTTTGGCTTTTAGTTATATGGATGATGTGGTATTGGGGGC6940.1449741595049989No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA6930.14476526302156228No Hit
ATGTTAATATGGGCCTGAAACTCAGACAAATATTGTGGTTTCATATTTCC6780.14163181577001333No Hit
GTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAAC6760.14121402280314013No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6730.14058733335283036No Hit
TCTTTGGAGTTACTCTCTTTTTTACCTTCTGACTTCTTTCCTTCCATTCG6600.13787167906815456No Hit
CTTACAGTTAATGAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTT6440.13452933533316902No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6420.13411154236629583No Hit
ATGTAATTGGAAGTTGGGGTACTTTACCACAAGAACATATTGTACAAAAA6400.1336937493994226No Hit
ACTTTATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAAGAACAT6390.133484852915986No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6340.13244037049880303No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6320.13202257753192984No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6290.13139588808162003No Hit
GGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG6270.13097809511474684No Hit
CTTCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTG6190.12930692324725407No Hit
ATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAG6100.12742685489632466No Hit
ATTTAAAGGAGGACATTATTAATAGATGTCAACAATATGTGGGCCCTCTT6080.12700906192945147No Hit
GCCCAAAAGACCCACAATTCTTTGACATACTTTCCAATCAATAGGTCTAT6070.12680016544601488No Hit
TTCCTCTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTC6040.12617347599570508No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6010.1255467865453953No Hit
GTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAA5990.1251289935785221No Hit
ACCCAGCATCCCGGGAAGCAGTAGTCAGCTATGTCAATGTTAATATGGGC5950.1242934076447757No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5870.12262223577728293No Hit
GGCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5860.12241333929384633No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT5790.12095106390979013No Hit
CAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGACTAAAATTAAT5770.12053327094291694No Hit
GTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACT5750.12011547797604374No Hit
TATCCAAGGGCAAATATTTGGTACGGGTAGGATAGAACCTAGCAGGCATA5740.11990658149260715No Hit
CCCCTATCCTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5710.11927989204229736No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5680.11865320259198757No Hit
AGAACATATTGTACAAAAAATCAAGCAATGTTTTCGAAAACTACCTGTAA5670.11844430610855097No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5660.11823540962511436No Hit
CCTTTAAATGAATGTGAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG5620.11739982369136798No Hit
CTGCTAGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5610.11719092720793138No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5610.11719092720793138No Hit
GGATAATACGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTACGGGT5570.116355341274185No Hit
ATACCACATCATCCATATAACTAAAAGCCAAACAGTGGGGGAAAGCCCTA5530.11551975534043861No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCAAGCTTACAGACCACCAAATGCC5530.11551975534043861No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5510.11510196237356542No Hit
GTTTAATGCCTTTATCCAAGGGCAAATATTTGGTACGGGTAGGATAGAAC5460.11405747995638241No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACTGTACCTGTC5440.11363968698950921No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5430.11343079050607262No Hit
CTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTG5410.11301299753919943No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC5390.11259520457232623No Hit
TAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTATGGGATATGTAA5370.11217741160545304No Hit
GGGTATACATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTA5370.11217741160545304No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCCTCTACTG5350.11175961863857983No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5310.11092403270483345No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5300.11071513622139685No Hit
CATTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAA5300.11071513622139685No Hit
TAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5290.11050623973796025No Hit
CTCTAAGGCCTCCCGATATAGAGCTGAGGCGGTGTCGAGGAGATCTCGAA5280.11029734325452366No Hit
ATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATTTTCAGGAT5270.11008844677108706No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA5170.10799948193672107No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC5160.10779058545328447No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGT5150.10758168896984788No Hit
GGATAGGGGCATTTGGTGGTCTGTAAGCTTGAGGAGTGCGAATCCACACT5130.10716389600297468No Hit
CAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAAGGAGACTAAAATT5060.10570162061891851No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT5060.10570162061891851No Hit
GTACAGTAGAGGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA5020.10486603468517211No Hit
CTGTAAGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTC5010.10465713820173551No Hit
CTTTCCAATCAATAGGTCTATTTACAGGTAGTTTTCGAAAACATTGCTTG4970.10382155226798913No Hit
GTACAATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4940.10319486281767934No Hit
GTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTC4900.10235927688393293No Hit
ACACAGAATAGCTTGCCTGAGTGCTGTATGGTGAGGTGAACAATGTTCCG4890.10215038040049633No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC4880.10194148391705973No Hit
GCGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA4870.10173258743362314No Hit
TAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT4870.10173258743362314No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4810.10047920853300356No Hit
GGATTAAAGACAGGTACAGTAGAGGAATAAAGCCCAGTAAAGTTTCCCAC4800.10027031204956696No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT4790.10006141556613037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)