FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451548
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA23710.5250826047286224No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21480.475696935873927No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC18140.4017291627911097No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTTAACAAGAAAAACCCC14580.3228892609423583No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12190.2699602257124381No Hit
CCTAATAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAAC10320.2285471312019985No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC10150.22478230442832212No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9910.21946725486548493No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9410.2083942349429075No Hit
CTCTACGACCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8610.1906774030667836No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8370.18536235350394642No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT8000.17716831876123912No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7660.16963866521388643No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT7620.16875282362008026No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT7570.16764552162782254No Hit
CTACAAACCTTGCCAGCAAATCCACCTCCTGCCTCTACCAATCGCCAGTC6630.14682824417337692No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6540.14483510058731297No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6280.1390771302275727No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6090.13486938265699328No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5960.13199039747712316No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5930.13132601628176852No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5830.129111412297253No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG5730.1268968083127375No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC5710.12645388751583442No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5680.12578950632047978No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5660.1253465855235767No Hit
GACTACTAGGTCCCTGGATGTTGGATCTTCCAAATTTCCACCCACCCAGG5540.12268906074215809No Hit
CTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5380.1191456943669333No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5380.1191456943669333No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA5200.11515940719480543No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5190.11493794679635388No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5090.11272334281183839No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT5000.11073019922577446No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5000.11073019922577446No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4930.1091799764366136No Hit
ACTGTGCAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG4890.10829413484280742No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4860.10762975364745277No Hit
GTCGTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4830.10696537245209813No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4830.10696537245209813No Hit
ACCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAATAGTTG4810.10652245165519501No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTACGA4800.10630099125674347No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCGCATTGGAAAACACCCTCTTTTC4790.10607953085829191No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGT4780.10585807045984036No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4740.10497222886603419No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGACTTAATGTCTCTA4650.10297908527997024No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4650.10297908527997024No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT4630.10253616448306713No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4590.10165032288926094No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)