FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences451548
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21260.4708248071079929No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA19210.42542542542542544No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC17580.38932738047782295No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTTAACAAGAAAAACCCC10880.2409489135152852No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9640.21348782410729314No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT9350.20706547255219823No Hit
CTCTATGGAAGGCGGGTGTATTATATAAGAGAGAAACAACACATAGCGCC9070.20086458139555485No Hit
CCTAATAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAAC8820.19532807143426614No Hit
CTCTACGACCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8560.18957010107452585No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7560.16742406122937098No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT7390.16365923445569464No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT6810.1508145313455048No Hit
ATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA6730.1490428481578924No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT6610.14638532337647384No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC6410.14195611540744282No Hit
CTCTTATATAATACACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT6030.13354062026628397No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5440.1204744567576426No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5310.11759547157777245No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT5110.11316626360874148No Hit
GACTACTAGGTCCCTGGATGTTGGATCTTCCAAATTTCCACCCACCCAGG4940.10940143683506516No Hit
GTCGTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC4870.10785121404590431No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4780.10585807045984036No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT4560.1009859416939063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)