FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487848

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487848
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences427324
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA28220.6603888384457695No Hit
GTGCTGGTGCGCACACCAATTTATGCCTACAGCCTCCTAATACAAACAAC20250.47387930469620243No Hit
CTCTAAGACCGTATCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA18090.42333217886194086No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC16670.39010212391534294No Hit
CATTTACACCAAGACATTATCAAAAAATGCGAACAATTTGTAGGCCCACT12540.29345414720446317No Hit
CCTTTATACCGCTGTTACCAATTTTCTTCTGTCTTTGGGTATACATTTAA10420.2438430792560212No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10310.24126892007001713No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9740.22793009519708698No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG9330.21833550186743547No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT8830.20663477829468974No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG8650.20242251780850126No Hit
CACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTTCACATTTCTT8310.19446602577903419No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC8310.19446602577903419No Hit
GTCTTAGAGTATTTGGTATCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8020.18767960610684165No Hit
ATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTACTGAAGGAAAGAAGT7920.18533946139229251No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTTCACCAAACTCTGCAAG7870.18416938903501792No Hit
ATCTAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGAGA7750.18136121537755895No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7590.17761698383428032No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG7530.17621289700555084No Hit
GTTAATATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC7310.17106457863354269No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7060.16521421684716983No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC7030.16451217343280508No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCTAGAAGATCTCGTA6840.1600658984751617No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG6710.15702371034624782No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6640.1553856090460634No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT6590.15421553668878882No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC6500.1521094064456946No Hit
CTCACAGTCAATGAAAAAAGAAGACTGCAATTGATTATGCCTGCTAGGTT6390.14953524725969053No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC6260.14649305913077665No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC6180.14462094335913733No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6090.1425148131160431No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC5970.1397066394585841No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5960.1394726249871292No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5740.1343243066151211No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC5740.1343243066151211No Hit
ATCCAAATGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5680.13292021978639157No Hit
CACCAAGACATTATCAAAAAATGCGAACAATTTGTAGGCCCACTCACAGT5620.1315161329576621No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5460.12777190141438347No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5450.12753788694292856No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5400.126367814585654No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT5370.12566577117128924No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5370.12566577117128924No Hit
GTTATGTTAACACTAATATGGGCCTAAAGTTTAGGCAACTATTGTGGTTT5300.12402766987110482No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5290.12379365539964991No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5230.12238956857092043No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5200.12168752515655569No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA5180.12121949621364586No Hit
CTCCGAAAGATACCAAATACTCTAAGACCGTATCTCTTCCAAAAGTGAGA5170.12098548174219095No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTTCCTGTC5150.1205174527992811No Hit
TTCTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC5140.12028343832782619No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT5100.11934738044200653No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGATACCAAATACTCTAAGA4950.11583716337018281No Hit
GCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCA4950.11583716337018281No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT4790.11209293182690416No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCA4760.11139088841253943No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4690.10975278711235503No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA4640.10858271475508047No Hit
CATTTAAATCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT4520.10577454109762148No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA4500.10530651215471165No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4490.10507249768325674No Hit
GTCTTTGGGTATACATTTAAATCCTAACAAAACAAAAAGATGGGGTTACT4440.10390242532598216No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGTTGCCT4440.10390242532598216No Hit
GGATAACCACATTGCGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4390.10273235296870759No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA4380.10249833849725266No Hit
GTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTTCTGTC4370.10226432402579776No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4350.10179629508288793No Hit
GTATGGATCGGCAGAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC4290.10039220825415844No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGCGTAAAAGGGGC4280.10015819378270353No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)