FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487850

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487850
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542474
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48170.8879688243123173No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA32500.5991070539786238No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12870.23724639337553505No Hit
CATTTACACCAAGACATCATTCAAAAATGTGAACAATTTGTAGGTCCACT12770.2354029870556008No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTGTTGTGGTTTCACATTTCTT11500.21199172679243614No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT11060.20388073898472553No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAAATTTCAT10910.2011156295048242No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTGAT10690.19706013560096888No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT10290.189686510321232No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC10240.18876480716126487No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9940.18323458820146218No Hit
CCCTTATCTAATGGCAAATATTTGGTGACCTTTGGATAAAACCTAGCAGG9820.18102250061754113No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG9340.1721741502818568No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTGACCTTTGGATAAAAC9160.1688560189059752No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9060.167012612586041No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9000.16590656879408044No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8820.16258843741819884No Hit
CAATAGGCCTGTTAACAGGAAGTTTCTTAAAACATTCTTTGATTTTCTGT8180.1507906369706198No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT8120.14968459317865923No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTGACCTT8120.14968459317865923No Hit
ACACTAGATACTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG8060.1485785493866987No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7990.14728816496274477No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7840.14452305548284342No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7720.14231096789892234No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT7710.14212662726692893No Hit
GACCCATACATTCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCA7550.13917717715503417No Hit
ATATAGAGCTGAGGCGGTATCAAGAAGATCTCGTACTGAAGGAAAGAAGT7510.13843981462706045No Hit
ACACCAGATACTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG7480.1378867927310802No Hit
GTATGGGTCATTGCCACAAGATCACATCAGACAGAAAATCAAAGAATGTT7380.13604338641114597No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7370.13585904577915253No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA7360.13567470514715912No Hit
GATATACATTTACACCAAGACATCATTCAAAAATGTGAACAATTTGTAGG7340.13530602388317228No Hit
TACCCTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7290.13438432072320516No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7200.13272525503526436No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7160.13198789250729068No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTGATATACATTTACA6860.126457673547488No Hit
GTATCAAGAAGATCTCGTACTGAAGGAAAGAAGTCAGAAGGCAAAAACGA6800.12535162975552747No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC6650.12258652027562611No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT6530.12037443269170503No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAC6520.12019009205971162No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6420.11834668573977739No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA6410.11816234510778398No Hit
CCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC6400.11797800447579053No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6300.11613459815585632No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6280.11576591689186946No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6180.11392251057193525No Hit
CATCATACAGAAAATCAAAGAATGTTTTAAGAAACTTCCTGTTAACAGGC6100.11244778551598787No Hit
CATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGTCAATGAGAAAC6080.11207910425200102No Hit
TCATCAAAAAATGTGAACAATTTGTAGGTCCACTCACAGTCAATGAGAAA6060.11171042298801417No Hit
ATACATTCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTT5990.11042003856406021No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT5990.11042003856406021No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC5860.10802361034814571No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTG5850.1078392697161523No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5810.1071019071881786No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA5810.1071019071881786No Hit
CTCTAAGGCTTCCCGATATAGAGCTGAGGCGGTATCAAGAAGATCTCGTA5770.10636454466020492No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC5750.10599586339621807No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5710.10525850086824438No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5660.10433679770827727No Hit
GTGTAAATGTATATCAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5630.103783775812297No Hit
AATTTAAAGAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATA5580.10286207265232988No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5530.10194036949236276No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5480.10101866633239565No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTGTACCTGTC5450.10046564443641538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)