FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences542474
Sequences flagged as poor quality0
Sequence length35-151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN48060.8859410773603896No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA31030.5720089810755907No Hit
CTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA12790.23577166831958768No Hit
CACTAATATGGGCCTAAAGTTCAGACAACTGTTGTGGTTTCACATTTCTT11490.2118073861604427No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTGACCTTTGGATAAAAC11410.21033266110449533No Hit
CATTTACACCAAGACATCATTCAAAAATGTGAACAATTTGTAGGTCCACT11300.20830491415256766No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC10990.20259035456077157No Hit
CCCTTATCTAATGGCAAATATTTGGTGACCTTTGGATAAAACCTAGCAGG10950.2018529920327979No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10800.19908788255289653No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTAAATTTCAT10680.19687579496897545No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACCACCGTG10040.18507799452139642No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA9910.18268156630548193No Hit
CTTTAGGACATGGACATTGACCCTTATAAAGAATTTGGAGCTACTGTGGA9890.18231288504149507No Hit
AGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9780.18028513808956742No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTGAT9750.17973211619358714No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9200.1695933814339489No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT9170.16904035953796864No Hit
GGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTGACCTT9160.1688560189059752No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8970.1653535468981002No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC8580.1581642622503567No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8260.15226536202656715No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8020.14784118685872502No Hit
CAATAGGCCTGTTAACAGGAAGTTTCTTAAAACATTCTTTGATTTTCTGT8000.14747250559473818No Hit
TTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTC7970.1469194836987579No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT7950.14655080243477106No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG7890.14544475864281053No Hit
ACACTAGATACTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG7570.139545858419021No Hit
ATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGA7540.13899283652304076No Hit
GTATGGGTCATTGCCACAAGATCACATCAGACAGAAAATCAAAGAATGTT7120.13125052997931697No Hit
GATATACATTTACACCAAGACATCATTCAAAAATGTGAACAATTTGTAGG6990.12885410176340248No Hit
GTTCTGGATAATAAGGTTTAATACCCTTATCTAATGGCAAATATTTGGTG6870.1266420141794814No Hit
ACACCAGATACTCTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATG6790.12516728912353403No Hit
GGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCATTGAC6780.12498294849154061No Hit
CATCATACAGAAAATCAAAGAATGTTTTAAGAAACTTCCTGTTAACAGGC6740.12424558596356693No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6680.12313954217160639No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6660.12277086090761954No Hit
GACCCATACATTCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCA6550.12074311395569189No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTGATATACATTTACA6370.11742498257981027No Hit
CTATAACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAC6150.11336948867595496No Hit
TAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGT6150.11336948867595496No Hit
ATATAGAGCTGAGGCGGTATCAAGAAGATCTCGTACTGAAGGAAAGAAGT6070.11189476362000761No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6070.11189476362000761No Hit
CCTTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCGTTTCTCA6010.11078871982804706No Hit
CTTATAAAGAATTTGGAGCTACTGTGGAGTTACTCTCGTTTTTGCCTTCT6000.11060437919605363No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5960.10986701666807995No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5920.10912965414010625No Hit
GTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGGGTTACT5800.10691756655618519No Hit
GTATAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACCACATC5770.10636454466020492No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5770.10636454466020492No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG5700.10507416023625095No Hit
GTCAATGAGAAACGAAGACTGCAATTAATTATGCCTGCTAGGTTTTATCC5700.10507416023625095No Hit
ATTTGTAGGTCCACTCACAGTCAATGAGAAACGAAGACTGCAATTAATTA5690.10488981960425753No Hit
CCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTC5660.10433679770827727No Hit
ATACATTCCAATGACATAGCCCATGAAATTTAAAGAGTAACCCCATCTTT5530.10194036949236276No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5510.10157168822837591No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTGACCTTTGGATAAAA5480.10101866633239565No Hit
GCTTTATTCTTCTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCT5450.10046564443641538No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)