FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450567
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA23010.5106898641045616No Hit
CATTTAAACCCTGCTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT19940.44255349370903774No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT19150.42502003031735563No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT16880.37463906588809215No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCGGG16410.3642077648829142No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTGATTATGCCTGCTAGG15610.34645235891665394No Hit
GTATAGGGAGGCCCTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAG14280.3169339964977462No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12340.27387713702956495No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA11640.2583411568090872No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11090.24613431520728327No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAGATTACAATCTA11020.24458071718523547No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10740.2383663250970444No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT10580.23481524390379233No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG10490.23281776073258803No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC10110.2243839428986144No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9990.2217206320036754No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9970.22127674685451887No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9750.21639401021379726No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG9530.2115112735730757No Hit
TCCCTATACAAAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA8980.19930443197127173No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT8880.19708500622548925No Hit
CAATAGGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTAT8650.19198032701018938No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8620.19131449928645464No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8420.18687564779488955No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACCACAGAGT8270.18354650917621573No Hit
GTATACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATC8060.17888571511007242No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7890.1751126913422421No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC7810.17333715074561606No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7780.17267132302188132No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7770.17244938044730307No Hit
GGTTAGGATAAAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCA7690.17067383985067702No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7660.17000801212694228No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7570.16801052895573798No Hit
GGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTATACAAT7550.16756664380658148No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA7520.16690081608284674No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7370.16357167746417292No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7320.16246196459128165No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT7190.15957671112176436No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC7170.15913282597260783No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7140.1584669982488731No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7080.15713534280140357No Hit
GTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTATACAATA7070.15691340022682532No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6980.15491591705562102No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6920.15358426160815153No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6710.14892346754200816No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6690.14847958239285167No Hit
GTTTAATACCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6680.14825763981827342No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGCAGGGTTTAAATGTATA6660.14781375466911692No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6520.14470655862502138No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG6480.14381878832670836No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6440.14293101802839533No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6410.14226519030466056No Hit
CTCTAGGGCCTCCCTATACAAAGCAGAGGCGGTGTCGAGGAGATCACGAA6390.14182130515550406No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6110.13560691306731296No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6090.13516302791815646No Hit
GGAACATATTGTATACAAAATCAAACAATGTTTTCGGAAACTTCCTATTA6040.1340533150452652No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5890.13072417642659137No Hit
TTGACATACTTTCCAATCAATAGGTCTATTAATAGGAAGTTTCCGAAAAC5880.13050223385201312No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG5850.12983640612827838No Hit
GCCCTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAGCACTAAGGCA5850.12983640612827838No Hit
AACATATTGTATACAAAATCAAACAATGTTTTCGGAAACTTCCTATTAAT5840.12961446355370013No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG5770.12806086553165233No Hit
ATTGTATACAAAATCAAACAATGTTTTCGGAAACTTCCTATTAATAGACC5650.12539755463671332No Hit
ACTCTGTGGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGC5600.12428784176382202No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCACTTGAAAG5600.12428784176382202No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5590.12406589918924377No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5560.12340007146550902No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5550.12317812889093077No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAATCTCCTCCCCC5530.12273424374177426No Hit
ATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTATACAATATGTT5520.12251230116719598No Hit
CCTTACCACAGGAACATATTGTATACAAAATCAAACAATGTTTTCGGAAA5510.12229035859261773No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5510.12229035859261773No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5440.12073676057056998No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT5430.12051481799599173No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5380.11940510512310044No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCGGGTCTTTGCCAAGTGTTTGCTG5370.1191831625485222No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5360.11896121997394395No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGCACCTGTC5340.11851733482478743No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTGCTAAA5310.11785150710105266No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5300.11762956452647441No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG5260.1167417942281614No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCG5250.11651985165358315No Hit
TATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA5220.11585402392984838No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5200.11541013878069188No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4970.11030545956539205No Hit
ATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAA4970.11030545956539205No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4930.10941768926707905No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAG4900.10875186154334426No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGTATTAAACCTTAT4870.10808603381960952No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4820.10697632094671823No Hit
AATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTATACAATATGTTCCT4790.10631049322298348No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG4780.10608855064840524No Hit
GCTTTGTATAGGGAGGCCCTAGAGTCTCCGGAGCATTGTTCACCTCACCA4770.10586660807382697No Hit
CTTGAAAGAGGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTA4740.10520078035009223No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTTACTACTAATTCCCGGG4720.1047568952009357No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC4700.1043130100517792No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCGGGTC4690.10409106747720094No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4690.10409106747720094No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG4680.10386912490262269No Hit
GGATAAAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCATTAAC4650.10320329717888795No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGGCAGACGAAGAAGG4580.10164969915684016No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4560.10120581400768365No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG4550.1009838714331054No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4540.10076192885852715No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG4530.1005399862839489No Hit
TGCTAGGTCTTCCAAATTACTTCCCACCCAGGTGGCCAGATTCATCAACT4520.10031804370937063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)