FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002487853

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487853
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences450567
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCGGG19750.4383365847920509No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19230.42679557091398174No Hit
CATTTAAACCCTGCTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT18730.4156984421850691No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17970.39883080651712177No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT17940.398164978793387No Hit
GTATAGGGAGGCCCTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAG15860.35200092328111027No Hit
CTCTTACAGTTAATGAAAAAAGAAGATTAAAATTGATTATGCCTGCTAGG15180.336908828209789No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA12460.27654044792450405No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11580.2570095013616177No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG11210.2487976261022223No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10850.24080769341740518No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAGATTACAATCTA10800.2396979805445139No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA10570.2345933013292141No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA9990.2217206320036754No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT9900.2197231488324711No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC9790.2172817805121103No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG9520.21128933099849745No Hit
ACCCATCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCG9000.19974831712042826No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT8730.19375586760681543No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8660.19220226958476763No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8660.19220226958476763No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8550.18976090126440684No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8520.1890950735406721No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG8420.18687564779488955No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8230.18265873887790274No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG8150.1808831982812767No Hit
GGTTAGGATAAAACCTAGCAGGCATAATCAATTTTAATCTTCTTTTTTCA7960.17666628936428988No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACCACAGAGT7920.17577851906597688No Hit
GTATACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATC7890.1751126913422421No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT7880.17489074876766386No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7600.16867635667947276No Hit
GGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTATACAAT7540.16734470123200323No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7540.16734470123200323No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG7370.16357167746417292No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7360.16334973488959467No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7350.16312779231501642No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG7350.16312779231501642No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA7270.16135225171839038No Hit
TCCCTATACAAAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA7160.15891088339802958No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7160.15891088339802958No Hit
GTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTATACAATA6930.15380620418272978No Hit
GTTTAATACCTTTATCTAAGGGCAAATATTTGGTAAGGTTAGGATAAAAC6820.15136483586236898No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6750.1498112378403212No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6750.1498112378403212No Hit
CAATAGGTCTATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTAT6670.14803569724369517No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGCAGGGTTTAAATGTATA6480.14381878832670836No Hit
GCCCTAGAGTCTCCGGAGCATTGTTCACCTCACCATACAGCACTAAGGCA6410.14226519030466056No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6410.14226519030466056No Hit
CTTCAAAGACTGTGTATTTAAAGACTGGGAGGAGTTGGGGGAGGAGATTA6400.1420432477300823No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6380.1415993625809258No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6260.13893605168598677No Hit
ACTCTGTGGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGC6240.13849216653683027No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC6180.13716051108936073No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6170.13693856851478248No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCGGGTCTTTGCCAAGTGTTTGCTG6060.13449720019442168No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT6050.13427525761984344No Hit
CTCTAGGGCCTCCCTATACAAAGCAGAGGCGGTGTCGAGGAGATCACGAA5980.13272165959779567No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCG5950.13205583187406092No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT5740.12739503780791758No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG5720.12695115265876106No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5600.12428784176382202No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG5590.12406589918924377No Hit
CTCTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGT5530.12273424374177426No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5470.12140258829430473No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5450.12095870314514823No Hit
CTTATATAAAATACCCGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT5420.12029287542141345No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5410.1200709328468352No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCACTTGAAAG5380.11940510512310044No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5360.11896121997394395No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5240.1162979090790049No Hit
CTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACCCTGCTAAA5170.11474431105695712No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAATCTCCTCCCCC5150.11430042590780062No Hit
GCTTTGTATAGGGAGGCCCTAGAGTCTCCGGAGCATTGTTCACCTCACCA5100.11319071303490934No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5070.11252488531117459No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5030.11163711501286158No Hit
GGAACATATTGTATACAAAATCAAACAATGTTTTCGGAAACTTCCTATTA5030.11163711501286158No Hit
AACATATTGTATACAAAATCAAACAATGTTTTCGGAAACTTCCTATTAAT4990.11074934471454856No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCGGGTC4980.11052740213997031No Hit
GGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACGGGGCG4960.1100835169908138No Hit
CCTTACCACAGGAACATATTGTATACAAAATCAAACAATGTTTTCGGAAA4920.10919574669250078No Hit
TTGACATACTTTCCAATCAATAGGTCTATTAATAGGAAGTTTCCGAAAAC4910.10897380411792253No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4780.10608855064840524No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4770.10586660807382697No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG4750.10542272292467048No Hit
CTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTTTG4720.1047568952009357No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4700.1043130100517792No Hit
GTATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4690.10409106747720094No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4690.10409106747720094No Hit
ATTGTATACAAAATCAAACAATGTTTTCGGAAACTTCCTATTAATAGACC4670.10364718232804444No Hit
ATTAATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTATACAATATGTT4610.10231552688057491No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGCACCTGTC4570.10142775658226191No Hit
TGGAAACCACCGTGAACGCCCGCCAGGTCTTGCCCAAGGTCTTACATAAG4530.1005399862839489No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)