FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487855

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487855
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences441152
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN29730.6739173799506746No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACGCTTCCGGA15220.34500580298853906No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC13870.3144041056143914No Hit
GTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10570.23959995647758595No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA8700.1972109386333962No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTAAAA8290.18791708980124763No Hit
CATCTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT8010.1815700710866096No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7740.17544973161178007No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7550.17114282605541856No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT7490.16978275061656753No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7100.160942260264036No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT6920.15686203394748294No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG6740.15278180763092994No Hit
CATGTATACAAGCTAAACAGGCTTTTACCTTCTCGCCAACTTACAAGTCC6620.1500616567532279No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAGCGAATTGTGGGTCT6610.14983497751341943No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6590.1493816190338024No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6570.1489282605541854No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6460.14643478891629189No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6300.14280792107935586No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATATGGTTTTGGGGGC6290.14258124183954737No Hit
CTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGGA6190.14031444944146235No Hit
GTAAACACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGC5900.1337407514870158No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5660.12830044973161178No Hit
GCTTTAGGGCATGGACATTGACACGTATAAAGAATTTGGAGCTTCTGTGG5660.12830044973161178No Hit
GCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGCTGACATACT5560.12603365733352676No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA5500.12467358189467576No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5220.11832656318003772No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA5200.11787320470042072No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5170.1171931669809952No Hit
CCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGACA5110.11583309154214419No Hit
CAGTTATATGGATGATATGGTTTTGGGGGCCAAGTCTGTACAACATCTTG5070.1149263745829102No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCACGCATGCGCTGATGGCC5060.1146996953431017No Hit
GTATACATCTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAAC5030.11401965762367619No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC4950.11220622370520818No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4930.11175286522559118No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4920.11152618598578269No Hit
GGATATCCACATTGCGTAAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4880.11061946902654868No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4860.11016611054693168No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT4860.11016611054693168No Hit
ACTCACAGCTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4810.10903271434788916No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA4770.10812599738865515No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACCTT4750.10767263890903815No Hit
GAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC4740.10744595966922965No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4740.10744595966922965No Hit
GTTATATGGATGATATGGTTTTGGGGGCCAAGTCTGTACAACATCTTGAG4740.10744595966922965No Hit
GGATAATACGGTTTTATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4680.10608588423037864No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4660.10563252575076165No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC4610.10449912955171914No Hit
ACTACATACTCTGGATAATACGGTTTTATCCCTTTATCTAAGGGCAAATA4590.10404577107210214No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4500.10200565791382561No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4480.10155229943420861No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4470.10132562019440011No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)