FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487856

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487856
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297837
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12810.43010102841487124No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11810.3965256163606268No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10470.3515345642079392No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9510.3193021686358646No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9120.3062077579347093No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA6820.22898431020994706No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6120.20548152177197596No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5990.2011167182049242No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5980.20078096408438173No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG5830.19574465227624507No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5810.19507314403516018No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5540.1860077827805142No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5540.1860077827805142No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA5260.17660666740532574No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATA5240.17593515916424085No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG4650.15612566605223663No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA4640.1557899119316942No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT4630.15545415781115174No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGCATACAAGCAAAAC4420.14840332127976041No Hit
GGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTAACATT4090.13732343530185975No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA4090.13732343530185975No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA4080.1369876811813173No Hit
TATCCACATTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG4010.1346374023375202No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG3880.1302725987704684No Hit
GTGCTGGACACAAGATATGTCTGTAAAAGGCAACAGCATTTCTCTACACT3880.1302725987704684No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3850.12926533640884108No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT3710.12456477872124685No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3700.12422902460070442No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG3670.12322176223907708No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3600.12087148339527996No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT3580.12019997515419509No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3450.1158351715871433No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3440.11549941746660086No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3420.11482790922551597No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3390.11382064686388862No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC3380.1134848927433462No Hit
ACATAGAGGTGCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG3380.1134848927433462No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3370.11314913862280375No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3350.11247763038171885No Hit
ACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTA3350.11247763038171885No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3350.11247763038171885No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3330.11180612214063397No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3320.11147036802009153No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT3160.10609830209141241No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3130.10509103972978508No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA3120.10475528560924263No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3050.10240500676544553No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3050.10240500676544553No Hit
GTGCTGGAGAAGGAAAAGGGATGAATAGGCAGAATGCACAGGATTTTTAG3040.10206925264490307No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA3000.1007262361627333No Hit
ATCCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA3000.1007262361627333No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGC2990.10039048204219087No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)