FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487857

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487857
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences297837
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11380.3820881891773017No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10760.36127143370367015No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9470.3179591521536948No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9310.3125870862250157No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9240.31023680738121856No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA6630.2226049819196406No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5980.20078096408438173No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5710.1917156028297357No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5460.18332174981617463No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG5330.17895694624912284No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGCACCTCTATG5050.1695558308739344No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5040.16922007675339196No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG4930.16552678142742505No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4640.1557899119316942No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG4510.1514251083646424No Hit
GTGCTGGTGTGATGGTGTCCTACTTTCTAGGTGGTGTCAAGAACTTTCTA4420.14840332127976041No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATA4120.13833069766348707No Hit
GTGCTGGACACAAGATATGTCTGTAAAAGGCAACAGCATTTCTCTACACT4020.13497315645806263No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA3990.1339658940964353No Hit
GGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTAACATT3960.13295863173480796No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT3930.13195136937318064No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGCATACAAGCAAAAC3920.13161561525263818No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT3830.1285938281677562No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAA3810.1279223199266713No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA3790.1272508116855864No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT3750.12590779520341663No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3720.1249005328417893No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA3640.12221449987744974No Hit
TATCCACATTGCGTGAAAGGGGCTGCAAACCCCAAAAGACCCACAATTCG3630.1218787457569073No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3600.12087148339527996No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA3440.11549941746660086No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3440.11549941746660086No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT3420.11482790922551597No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG3340.11214187626117642No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC3300.11079885977900664No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC3270.10979159741737932No Hit
ACTCTGGATAATACGGTTTGATCCCTTTGTCTAAGGGCAAATATTTAGTA3210.10777707269412463No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA3160.10609830209141241No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT3100.10408377736815776No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3090.1037480232476153No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA3060.10274076088598798No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3000.1007262361627333No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT2980.10005472792164842No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)