FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002487858

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002487858
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471771
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG24890.5275864773375218No Hit
GGATATTATCAACAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG16630.3525015314633583No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT15320.3247338221298045No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT14780.31328759080146934No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14140.299721687004924No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13660.28954725915751495No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG13310.28212840551877927No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC12980.2751334863736855No Hit
ATTATCAACAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACGAAAA12490.2647470912794555No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT12040.2552085651725095No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATCTTGTGTACAATATGTT11770.249485449508342No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11680.24757774428695278No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC11070.23464774223087048No Hit
GTTTAGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGCA10390.22023396944704107No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC10160.21535872277015758No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG9870.20921167261234794No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT9770.20709200014413773No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9710.20582019666321158No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9190.1947978998285185No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTGTTGATAATATCCTCTTTC8960.189922653151635No Hit
ATGTAAGACCTTGGGCAAGACCTGTTGGGCGTTCACGGTGGTCTCCATGC8780.18610724270885662No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8710.18462347198110948No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8710.18462347198110948No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTC8630.1829277340065413No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCG8600.18229183226607823No Hit
ACAATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGA8460.17932429081058396No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT8210.1740251096400584No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATG8200.1738131423932374No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8180.17338920789959536No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8180.17338920789959536No Hit
GTACAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCC8170.17317724065277432No Hit
AAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTA8090.17148150267820617No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7870.1668182232481437No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA7790.16512248527357554No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTC7730.1638506817926494No Hit
GGATAAAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC7510.15918740236258694No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA7400.15685576264755569No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7390.15664379540073467No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCAAACTCCTCCCCCA7200.15261641771113527No Hit
CTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG7140.15134461423020915No Hit
CTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTGTACCTGTC7020.14880100726835688No Hit
GGGTAAAGGTTCAGATATTGTTTACATAGAAAGGCCTTGTAAGTTGGCGA6980.14795313828107282No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6890.14604543305968362No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6830.14477362957875747No Hit
GAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCA6790.14392576059147338No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6490.13756674318684275No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6490.13756674318684275No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6410.1358710052122746No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6410.1358710052122746No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6390.13544707071863255No Hit
GGCCTACATATTGTTGACATCTGTTGATAATATCCTCTTTCAAATGAATG6350.13459920173134848No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATCTTGTGTACAAT6230.13205559476949622No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACCGGGCTTTATTCTTCTACTG6170.1307837912885701No Hit
GATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGC6050.12824018432671783No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5940.1259085446116866No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5860.12421280663711842No Hit
GTTTGGAAGTAATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCC5860.12421280663711842No Hit
GTTTTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC5710.12103329793480312No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5620.11912559271341393No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT5610.11891362546659291No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG5600.11870165821977188No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5520.1170059202452037No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5510.1167939529983827No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5480.11615805125791963No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5470.1159460840110986No Hit
ATGATTAACTATGTGTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGG5440.11531018227063554No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5420.1148862477769935No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC5410.11467428053017246No Hit
GTTAATCATTACTTCCAAACTAGGCATTATTTACATACTCTGTGGAAGGC5400.11446231328335146No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5380.11403837878970942No Hit
AGTCTGCACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT5370.11382641154288839No Hit
GTCTTACATAAGAGGACTCTTGGACTCTTACATAAGAGGACTCTTGGACT5330.1129785425556043No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5300.11234264081514125No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG5290.11213067356832021No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGACCGGCAGATGAGAAGGCACAG5230.11085887008739409No Hit
GTTGATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCC5230.11085887008739409No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5190.11001100110011No Hit
ACATATTGTTGACATCTGTTGATAATATCCTCTTTCAAATGAATGTGAGG5190.11001100110011No Hit
ATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGA5150.10916313211282593No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5090.10789132863189979No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA5020.10640755790415266No Hit
GAATTTTTGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCC5000.10598362341051061No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG4980.10555968891686858No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4890.10365198369547937No Hit
ATGTGGTATTGGGGGCCAAGTCTGCACAACATCTTGAATCCCTTTTTACC4870.10322804920183733No Hit
ATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAG4840.10259214746137428No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGG4800.10174427847409018No Hit
CTCAAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA4770.10110837673362712No Hit
GTCCAAGAGTCCTCTTATGTAAGAGTCCAAGAGTCCTCTTATGTAAGACC4760.10089640948680609No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4750.10068444223998507No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4750.10068444223998507No Hit
CGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGGCAGACGAAGAAGG4740.10047247499316404No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4730.10026050774634304No Hit
ATATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTA4720.100048540499522No Hit
GTCTGCACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTTT4720.100048540499522No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)